identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
6C59D4C7E1AF5732BDD602F00DCC1623.text	6C59D4C7E1AF5732BDD602F00DCC1623.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Nigrograna asexualis L. Lu & Tibpromma 2022	<div><p>Nigrograna asexualis L. Lu &amp; Tibpromma sp. nov.</p><p>Fig. 4</p><p>Etymology.</p><p>The species epithet ' Nigrograna asexualis ' refers to the asexual morph.</p><p>Holotype.</p><p>ZHKU 22-0123.</p><p>Description.</p><p>Saprobic on decaying branch of Coffea arabica. Sexual morph: Undetermined. Asexual morph: Coelomycetous. Pycnidia 100-230 µm high, 120-180 µm wide (x̄ = 156 × 144 µm, n = 10), globose to subglobose, or pyriform, immersed, solitary, unilocular, dark brown, papillate ostiole, appearing as black spots on host surface. Pycnidial wall 11-16 µm wide (x̄ = 14 µm, n = 15), brown (#937463), the wall with pseudoparenchymatous cells. Conidiophores arising from the pycnidial wall, up to 46 µm long and 3-4.4 µm wide (x̄ = 3.4 µm, n = 25), filiform, septate, hyaline, simple to sparsely branched, with pegs along one or two sides and solitary phialides terminally. Phialides 3-6 × 1-2 µm (x̄ = 4.5 × 1.5 µm, n = 15), variable in shape, phialidic, discrete, ampulliform-lageniform-subcylindrical. Conidia 5-6.5 × 3-4 µm (x̄ = 5.5 × 3.7 µm, n = 30), ellipsoidal, unicellular, aseptate with 1-2 granules, subhyaline, smooth-walled.</p><p>Culture characteristics.</p><p>Conidium germinated on PDA within 24 h. Colonies growing on PDA reaching 5 cm diam. after two months at room temperature (22-26 °C). Colony dense, circular, surface sparsely hairy, radially striate, with a fimbriate edge, yellowish (#eabf83) to pale brown (#e1af33) at the center and dark brown (#6e5031) at the margin, reverse dark brown (#6e5031).</p><p>Material examined.</p><p><a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=101.01639&amp;materialsCitation.latitude=22.600555" title="Search Plazi for locations around (long 101.01639/lat 22.600555)">Pu'er City</a>, Yunnan Province, China, on a decaying branch of Coffea arabica, (22°36'2"N, 101°0'59"E, 1016.43 m), 16 September 2021, LiLu, Puer 1-14 (ZHKU 22-0123, holotype), ZHKUCC 22-0214 = ZHKUCC 22-0215. GenBank number; ITS: OP450965, LSU: OP450971, rpb 2: OP432241, SSU: OP450979, tef 1-α: OP432245 (ZHKUCC 22-0214, ex-type); ITS: OP450966, LSU: OP450972, rpb 2: OP432242, SSU: OP450980, tef 1-α: OP432246 (ZHKUCC 22-0215) .</p><p>Notes.</p><p>In multi-gene phylogeny, Nigrograna asexualis formed a separate (68% ML, 0.97 BIPP) and distinct clade within Nigrograna (Fig. 1). Morphologically, N. asexualis conforms to the morphological characteristics of Nigrograna by having hyaline or subhyaline, long and branched conidiophores, solitary phialides, and aseptate, ellipsoidal or cylindrical conidia (Jaklitsch and Voglmayr 2016; Dayarathne et al. 2020; Wanasinghe et al. 2020). Blast results of the sequences show that ITS is similar to N. fuscidula with 89% (MH856004), and SSU is similar to N. mycophila with 99.8% (KX650510). Nigrograna asexualis is different from N. fuscidula and N. mycophila by its ellipsoidal conidia, but the similarities of these three species are hyaline, 1-celled, smooth-walled conidia forming on philipides (Jaklitsch and Voglmayr 2016). The LSU and rpb 2 sequences of our strain blast results are similar to N. obliqua, and the similarities are 98.9% (KX650560) and 87% (KX650579) respectively, but N. obliqua lacks the asexual morph (Jaklitsch and Voglmayr 2016). The tef 1-α sequence of our strain is 95.8% (MF939615) similar to N. locuta-pollinis, which was isolated from hive-stored pollen of Brassica campestris L. that lacks morphology (Zhao et al. 2018). Therefore, we introduce N. asexualis as a distinct new species from coffee in China.</p></div>	https://treatment.plazi.org/id/6C59D4C7E1AF5732BDD602F00DCC1623	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Lu, Li;Karunarathna, Samantha C.;Dai, Dong-qin;Jayawardena, Ruvishika S.;Suwannarach, Nakarin;Tibpromma, Saowaluck	Lu, Li, Karunarathna, Samantha C., Dai, Dong-qin, Jayawardena, Ruvishika S., Suwannarach, Nakarin, Tibpromma, Saowaluck (2022): Three new species of Nigrograna (Dothideomycetes, Pleosporales) associated with Arabica coffee from Yunnan Province, China. MycoKeys 94: 51-71, DOI: http://dx.doi.org/10.3897/mycokeys.94.95751, URL: http://dx.doi.org/10.3897/mycokeys.94.95751
EB69069BB814520BBFD44E61265EAB85.text	EB69069BB814520BBFD44E61265EAB85.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Nigrograna coffeae L. Lu & Tibpromma 2022	<div><p>Nigrograna coffeae L. Lu &amp; Tibpromma sp. nov.</p><p>Fig. 2</p><p>Etymology.</p><p>Species epithet refers to the host genus " Coffea " where the fungus was isolated.</p><p>Holotype.</p><p>ZHKU 22-0121.</p><p>Description.</p><p>Saprobic on decaying branch of Coffea arabica . Sexual morph: Ascomata 90-140 µm high, 140-200 μm wide (x̄ = 115 × 168 μm, n = 10), immersed, solitary, black spots on substrate, subglobose to oval, sometimes obpyriform, some with ostiolate. Peridium 10-15 µm wide, composed of 3-5 layers, hyaline to brown (#937463) cells of textura angularis. Hamathecium 1.5-3 μm wide, composed of numerous, hyaline, filamentous, septate, branched, pseudoparaphyses. Asci 50-70 × 7-11 μm (x̄ = 58 × 9 μm, n = 20), 8-spored, bitunicate, fissitunicate, clavate to cylindric-clavate, short stalked, some with club-shape pedicel, apically rounded, with a small ocular chamber. Ascospores 12-16 × 4-5 μm, (x̄ = 14.4 × 4.6 μm, n = 30), overlapping uni- to bi-seriately arranged, fusiform, straight or slightly curved, hyaline when immature and become pale brown (#e1af33) to dark-brown (#6e5031) when mature, mostly 1-septate, few 2 or 3-septate, constricted at each septum, with obviously guttulate. Asexual morph: Undetermined.</p><p>Culture characteristics.</p><p>Ascospores germinated on PDA within 24 h and germ tubes arising from both ends. Colonies on PDA, reaching 4.5 cm diam. after two months of incubation at room temperature (22-26 °C), initially white (#f2f3f4) becoming grey (#bbbeb2) to dark brown (#6e5031) at maturity, dense, circular, slightly raised, smooth surface, radially fimbriate at the edge, reverse dark green (#3a4543) to brown (#937463).</p><p>Material examined.</p><p><a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=101.045555&amp;materialsCitation.latitude=22.521666" title="Search Plazi for locations around (long 101.045555/lat 22.521666)">Pu'wen Town</a>, Xishuangbanna, Yunnan Province, China, on a decaying branch of Coffea arabica, (22°31'18"N, 101°2'44"E, 856.89 m), 15 September 2021, LiLu, JHPW16 (ZHKU 22-0121, holotype), ZHKUCC 22-0210 = ZHKUCC 22-0211. GenBank number; ITS: OP450967, LSU: OP450973, rpb 2: OP432243, SSU: OP450981, tef 1-α: OP432247 (ZHKUCC 22-0210, ex-type); ITS: OP450968, LSU: OP450974, rpb 2: OP432244, SSU: OP450982, tef 1-α: OP432248 (ZHKUCC 22-0211) .</p><p>Notes.</p><p>Our phylogenetic analyses showed that Nigrograna coffeae forms an independent clade (100% ML, 1.00 BIPP, Fig. 1), and is phylogenetically related to N. yasuniana and N. jinghongensis . Nigrograna yasuniana was reported as endophytes from Conceveiba guianensis Aubl. in Ecuador, but there were not enough morphological data, the comparison of base pairs in ITS showed 3.4% differences (15/433 bp), LSU showed 1.5% differences (12/812bp), SSU only showed 0.3% differences (3/1028 bp), rpb 2 showed 14% differences (117/829 bp), and tef 1-α showed 3.2% differences (31/954 bp) ( Kolařík et al. 2017). Nigrograna jinghongensis was introduced as a saprobic fungus from woody litter in China, and our new isolate shares a similar size (12-16 × 4-5 μm vs 12-15 × 4-5.5 µm) and color (hyaline to dark brown vs yellowish-brown to brown) of ascospores with N. jinghongensis (Boonmee et al. 2021), but there are some significant differences in the size of the ascomata (90-140 µm high, 140-200 μm wide vs 300-400 µm high 220-300 μm wide) and the shape of ascospores (fusiform, straight or slightly curved vs ellipsoid) (Boonmee et al. 2021). Based on the sequence blast results, ITS, LSU and rpb 2 gene sequences were similar to Nigrograna sp., with 97.5% (MZ270683), 98.4% (MK762716), and 86% (MZ508421) respectively, SSU was similar to N. mycophila with 99% (KX650510), and tef 1-α was similar to N. yasuniana with 96.6% (LN626670). Therefore, we introduce our new isolate as a new species N. coffeae based on both morphological characteristics and phylogenetic analyses.</p></div>	https://treatment.plazi.org/id/EB69069BB814520BBFD44E61265EAB85	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Lu, Li;Karunarathna, Samantha C.;Dai, Dong-qin;Jayawardena, Ruvishika S.;Suwannarach, Nakarin;Tibpromma, Saowaluck	Lu, Li, Karunarathna, Samantha C., Dai, Dong-qin, Jayawardena, Ruvishika S., Suwannarach, Nakarin, Tibpromma, Saowaluck (2022): Three new species of Nigrograna (Dothideomycetes, Pleosporales) associated with Arabica coffee from Yunnan Province, China. MycoKeys 94: 51-71, DOI: http://dx.doi.org/10.3897/mycokeys.94.95751, URL: http://dx.doi.org/10.3897/mycokeys.94.95751
1B95946CF0165EA6988D8E1715C78A93.text	1B95946CF0165EA6988D8E1715C78A93.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Nigrograna puerensis L. Lu & Tibpromma 2022	<div><p>Nigrograna puerensis L. Lu &amp; Tibpromma sp. nov.</p><p>Fig. 3</p><p>Etymology.</p><p>The specific epithet " Nigrograna puerensis " refers to the location Pu’er City, where the type species was collected.</p><p>Holotype.</p><p>ZHKU 22-0122.</p><p>Description.</p><p>Saprobic on decaying branch of Coffea arabica . Sexual morph: Ascomata 90-180 µm high, 90-150 μm wide (x̄ = 138 × 115 μm, n = 10), immersed, with only ostiolar necks visible on the host surface or erumpent, solitary, subglobose to ellipsoid, dark brown (#6e5031). Peridium 10-15 μm wide (x̄ = 13 μm, n = 15), outer layer consists of 2-3 layers of textura prismatica, brown (#937463) and thick-walled cells, inner layer hyaline with thin-walled cells. Hamathecium composed of numerous, 1.5-2 µm wide (x̄ = 1.8 μm, n = 20), filamentous, hyaline, septate, pseudoparaphyse. Asci 50-80 × 8-11 μm (x̄ = 66 × 9.5 μm, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical to clavate, short pedicellate, apically rounded, with poorly developed ocular chamber. Ascospores 15-18 × 4-5 μm, (x̄ = 16 × 4.5 μm, n = 30), uni- to bi-seriately arranged, fusoid, apical cell and basal cell acute, and apical cell slightly wider than basal cell, straight or slightly curved, 1-septate, constricted at septum, guttulate, hyaline to yellow-brownish (#daceb8) when young, brownish (#937463) when mature. Asexual morph: Undetermined.</p><p>Culture characteristics.</p><p>On PDA, colonies reached up to 4 cm diam. after two months at room temperature (22-26 °C). Colony dense, circular, slightly raised at the center, surface with white aerial mycelium, fluffy, with a serrate edge, grayish (#c9bfb3) to dark brown (#6e5031) from center to edge, reverse dark green (#3a4543) to dark brown (#6e5031).</p><p>Material examined.</p><p><a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=101.01639&amp;materialsCitation.latitude=22.600555" title="Search Plazi for locations around (long 101.01639/lat 22.600555)">Pu'er City</a>, Yunnan Province, China, on a decaying branch of Coffea arabica, (22°36'2"N, 101°0'59"E, 1016.43 m), 16 September 2021, LiLu, Puer 1-4 (ZHKU 22-0122, holotype), ZHKUCC 22-0212 = ZHKUCC 22-0213. GenBank number; ITS: OP450969, LSU: OP450975, SSU: OP450983, tef 1-α: OP432249 (ZHKUCC 22-0212, ex-type); ITS: OP450970, LSU: OP450976, SSU: OP450984, tef 1-α: OP432250 (ZHKUCC 22-0213) .</p><p>Notes.</p><p>Nigrograna puerensis clusters with N. carollii with significant statistical support from ML 100% and BIPP 1.00. In morphology, our new strains best fit Nigrograna by having immersed ascomata, clavate and short pedicellate asci, and pale to brown, fusoid to narrowly ellipsoid, and septate ascospores (Jaklitsch and Voglmayr 2016; Zhang et al. 2020). Blast search results of ITS, LSU and tef 1-α sequence data revealed that our taxon (ZHKUCC 22-0212) is similar to N. mackinnonii (96% MZ270697, 99% KJ605422, and 95% LT797087 respectively), while the similarity of SSU sequence to N. carollii is as high as 99%. Based on nucleotide comparisons, our isolate (ZHKUCC 22-0212) differs from N. carollii (CCF 4484) by 9/490 bp (1.8%) in ITS, 2/222 bp (1%) in LSU, 2/1306 bp (0.2%) in SSU, and 10/530 bp (2%) in tef 1-α. Unfortunately, for N. carollii, sufficient morphological data was not available to compare with our novel taxon which was isolated as an endophyte on living sapwood of wild Hevea brasiliensis Müll . Arg., and N. mackinnonii which was isolated as a human pathogen (de Gruyter 2012; Kolařík et al. 2017). In addition, the colony morphology of N. carollii on PDA is described as colonies plane, effuse, and light gray ( Kolařík et al. 2017), while N. puerensis colony surface is seen as white aerial mycelium, fluffy, with a serrate edge, and grayish to dark brown from center to edge. Therefore, based on morphological and phylogenetic analyses, we introduce N. puerensis as a distinct new species.</p></div>	https://treatment.plazi.org/id/1B95946CF0165EA6988D8E1715C78A93	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Lu, Li;Karunarathna, Samantha C.;Dai, Dong-qin;Jayawardena, Ruvishika S.;Suwannarach, Nakarin;Tibpromma, Saowaluck	Lu, Li, Karunarathna, Samantha C., Dai, Dong-qin, Jayawardena, Ruvishika S., Suwannarach, Nakarin, Tibpromma, Saowaluck (2022): Three new species of Nigrograna (Dothideomycetes, Pleosporales) associated with Arabica coffee from Yunnan Province, China. MycoKeys 94: 51-71, DOI: http://dx.doi.org/10.3897/mycokeys.94.95751, URL: http://dx.doi.org/10.3897/mycokeys.94.95751
