taxonID	type	description	language	source
4E7587893A71FFD887CC8701FC797C2C.taxon	description	(Figure 1 A – C) Material examined Holotype ATBI slide numbers 13 apr 03 - 08 - 50 (USNM 1114530) and 2 paratypes (ATBI slide numbers 13 apr 03 - 7 - 9, 13 apr 03 - 7 - 16): North America, USA, Tennessee, Blount County, Great Smoky Mountains National Park, stream periphyton. Holotype and 2 paratypes (ATBI slide numbers 13 apr 03 - 08 - 50, 13 apr 03 - 7 - 9, 13 apr 03 - 7 - 16) from periphyton in a high-gradient section of Abram’s Creek upstream of limestone bedrock influences (35 ° 36.27261249 N, 83 ° 46.30223239 W, 520 m above sea level, 13 April 2003); leg. P. J. Bartels. A map indicating collecting sites is provided in Figure 5. Description (measurements of the holotype, USNM 1114530) Body length 298.0 (Figure 1 A). Body colour unknown, eyes present. Cuticle smooth. Mouth anteroventral. Bucco-pharyngeal apparatus of the Doryphoribius type: peribuccal lamellae and papulae absent, ventral lamina present (Figure 1 B). Oral cavity armature absent or not visible in PCM / DIC. Buccal tube 30.2 long and 3.5 [11.6] (external) wide; 2.8 [9.3] (internal) wide. Ventral lamina 18.6 [61.6] long. At the end of the buccal tube, triangular pharyngeal apophyses present. Stylet supports inserted on buccal tube at 22.0 [72.8]. Pharyngeal bulb spherical (31.3 X 31.3) with three granular macroplacoids [length configuration 1 (shortest) – 2 (longer) – 3 (longest)]. Microplacoid and septulum absent. First macroplacoid, 2.3 [7.6] long; second 2.5 [8.3] long; and third 3.0 [9.9] long. Macroplacoid row 10.1 [33.4] long. Claws of the Isohypsibius type, similar in size and shape (Figure 1 C). Primary branches of claws with well-developed accessory points. Lunules and other cuticular thickenings on legs absent. Claw lengths: leg I: external: basal claw 7.1 [23.5], primary branch 12.2 [40.4], secondary branch 10.6 [35.1]; leg IV: posterior (internal): basal claw 7.8 [25.8], primary branch 12.3 [40.7], secondary branch 10.6 [35.1]. Other claws not measurable. Eggs unknown. Remarks Measurements and pt values of selected morphological structures for all specimens are given in Table 1. Etymology The name ‘‘ longistipes ’’ refers to the length of the secondary claw branches; longi 5 long, stipes 5 branch. Type depositories The holotype (USNM 1114530) is deposited in the National Museum of Natural History, Smithsonian Institution, Washington, DC, USA. One paratype (ATBI 13 apr 03 - 7 - 9) is deposited in the Nelson Collection, ETSU, Johnson City, TN, USA, and one (ATBI 13 apr 03 - 7 - 16) is in the Department of Animal Taxonomy and Ecology, A. Mickiewicz University, Umultowska 89, Poznań, Poland. Differential diagnosis	en	Bartels, P. J., Nelson, D. R., Kaczmarek, Ł., Michalczyk, Ł. (2008): Three new species and one new record of the genus Doryphoribius Pilato, 1969 (Tardigrada: Eutardigrada: Hypsibiidae) from the Great Smoky Mountains National Park (Tennessee, USA). Journal of Natural History 42 (41 - 42): 2595-2608, DOI: 10.1080/00222930802357343, URL: http://dx.doi.org/10.1080/00222930802357343
4E7587893A71FFD887CC8701FC797C2C.taxon	description	It differs from D. qinlingense by: the absence of the oral cavity armature, a slightly higher pt of the stylet-support insertion point (71.7 – 72.8 in D. longistipes and 66.7 – 71.4 in D. qinlingense), a slightly higher pt of the buccal tube width (10.4 – 11.6 in D. longistipes and 7.3 – 10.0 in D. qinlingense), and a higher ratio of the secondary to the primary branches (80.0 – 86.9 in D. longistipes sp. nov. and about 68.5 in D. qinlingense). Doryphoribius minimus sp. nov. (Figures 2 A, B, 3 A – C) Material examined Holotype ATBI slide number 5 sept 04 - 12 - 46 (USNM 1114529) and 4 paratypes (ATBI slide numbers 5 sept 04 - 5 - 12, 5 sept 04 - 12 - 15, 5 sept 04 - 12 - 45, 5 sept 04 - 5 - 43): North America, USA, Tennessee, Blount County, Great Smoky Mountains National Park, lichen on dolomitic limestone rock. Holotype and 2 paratypes (ATBI slide numbers 5 sept 04 - 12 - 46, 5 sept 04 - 12 - 15, and 5 sept 04 - 12 - 45) from lichen on external wall of dolomitic limestone cave (Bull Cave, 35 ° 38.7759 N, 83 ° 48.4179 W, 544 m above sea level, 5 September 2004); 2 paratypes (ATBI slides 5 sept 04 - 5 - 12 and 5 sept 04 - 5 - 43) from lichen on external wall of dolomitic limestone cave (Gregory’s Cave, 35 ° 36.6789 N, 83 ° 48.3569 W, 584 m above sea level, 5 September 2004), leg. P. J. Bartels. A map indicating the collecting sites is provided in Figure 5. Description (measurements of the holotype, USNM 1114529) Body length 194.0 (Figure 2 A, B). Body colour unknown, eyes absent. Cuticle with rather large polygonal tubercles (diameter 1.3 – 4.0) on dorsal and lateral side of the body including legs (Figures 2 A, 3 A). Besides tubercles, eight rows of indistinct gibbosities are present on dorsolateral side of the body. Two gibbosities present in each row. One gibbosity on each side of the body (dorsal cuticle sensu stricto devoid of gibbosities). Mouth anteroventral. Bucco-pharyngeal apparatus of the Doryphoribius type: peribuccal lamellae and papulae absent, ventral lamina present (Figure 3 B). Oral cavity armature absent or not visible in PCM / DIC. Buccal tube 26.8 long and 1.9 [7.1] (external) wide; 1.0 [3.7] (internal) wide. Ventral lamina 15.3 [57.1] long. At the end of the buccal tube, triangular pharyngeal apophyses present. Stylet supports inserted on buccal tube at 18.5 [69.0]. Pharyngeal bulb with three granular macroplacoids [length configuration 1 (shortest) – 2 (longer) – 3 (longest)]. Microplacoid and septulum absent. First macroplacoid 1.6 [6.0] long; second 1.9 [7.1] long; third 2.4 [9.0]. Macroplacoid row 7.7 [28.7] long. Claws of the Isohypsibius type, similar in size and shape (Figure 3 C). Primary branches of claws with well-developed accessory points. Lunules and other cuticular thickenings on legs absent. Claw lengths: leg II: external: basal claw 3.6 [13.4], primary branch 8.7 [32.5], secondary branch 5.2 [19.4], internal primary branch 7.6 [28.4], secondary branch 4.4 [16.4]; leg IV: anterior (external): basal claw 3.0 [11.2], secondary branch 4.4 [16.4]; posterior (internal): basal claw 3.1 [11.6], primary branch 7.0 [26.1], secondary branch 4.9 [18.9]. Other claws not measurable. Eggs unknown. Remarks Measurements and pt values of selected morphological structures for all specimens are given in Table 2. Etymology The name ‘ minimus ’ refers to the small size of individuals of the new species. Type depositories The holotype (ATBI slide 5 sep 04 - 12 - 46) (USNM 1114529) is deposited in the National Museum of Natural History, Smithsonian Institution, Washington, DC, USA. One paratype (ATBI 5 sept 04 - 5 - 12) is deposited in the GSMNP collection, two (ATBI 5 sept 04 - 12 - 15 and 5 sept 04 - 5 - 43) are in the Nelson Collection, ETSU, Johnson City, TN, USA, and one (ATBI 5 sept 04 - 12 - 45) is in the Department of Animal Taxonomy and Ecology, A. Mickiewicz University, Umultowska 89, Poznań, Poland. Differential diagnosis	en	Bartels, P. J., Nelson, D. R., Kaczmarek, Ł., Michalczyk, Ł. (2008): Three new species and one new record of the genus Doryphoribius Pilato, 1969 (Tardigrada: Eutardigrada: Hypsibiidae) from the Great Smoky Mountains National Park (Tennessee, USA). Journal of Natural History 42 (41 - 42): 2595-2608, DOI: 10.1080/00222930802357343, URL: http://dx.doi.org/10.1080/00222930802357343
4E7587893A71FFD887CC8701FC797C2C.taxon	description	It differs from D. gibber by: a lower number of rows with gibbosities (10 in D. gibber and eight in D. minimus sp. nov.), a different gibbosities formula (4, 4, 4, 4, 4, 4, 4, 2, 2, 3 in D. gibber and 2 gibbosities in each row in D. minimus sp. nov.), a slightly smaller body size (220.0 – 273.0 in D. gibber and 151.1 – 200.0 in D. minimus sp. nov.), a different configuration of macroplacoids (generally 2 - 1 - 3 in D. gibber and 1 - 2 - 3 in D. minimus sp. nov.), and the absence of eyes. It differs from D. mariae by: a lower number of rows with gibbosities (12 in D. mariae and eight in D. minimus sp. nov.), a different gibbosities formula (four or six gibbosities in rows 1 – 11 and one gibbosity in row 12 in D. mariae and 2 gibbosities in each row in D. minimus sp. nov.), a different configuration of macroplacoids (generally 2 - 1 - 3 in D. vietnamensis and 1 - 2 - 3 in D. minimus sp. nov.), the absence of the oral cavity armature (two small dorsal teeth present in D. mariae), and the absence of eyes. Doryphoribius tergumrudis sp. nov. (Figure 4 A – D) Material examined Holotype (USNM 1114531) (ATBI slide number 13 apr 03 - 15 - 1): North America, USA, Tennessee, Blount County, Great Smoky Mountains National Park, stream periphyton. Holotype from stream periphyton in low-gradient Abram’s Creek in the limestone-based Cades Cove area of the GSMNP (35 ° 35.7079 N, 83 ° 50.4769 W, 520 m above sea level, 13.04.2003), leg. P. J. Bartels. A map indicating collecting sites is provided in Figure 5. Description (measurements of the holotype, USNM 1114531) Body length 519.0 (Figure 4 A). Body colour unknown, eyes absent. Cuticle smooth. Cuticle on legs I – III smooth and with large and evident granulation on legs IV (Figure 4 D). Granules large and round (diameter 1.0 – 1.8). Mouth anteroventral. Bucco-pharyngeal apparatus of the Doryphoribius type: peribuccal lamellae and papulae absent, ventral lamina present (Figure 4 B). Oral cavity armature absent or not visible in PCM / DIC. Buccal tube 43.8 long and 6.6 [15.1] (external) wide; 5.2 [11.9] (internal) wide. Ventral lamina 28.4 [64.8] long. At the end of the buccal tube, triangular pharyngeal apophyses present. Stylet supports inserted on buccal tube at 32.2 [73. 5]. Pharyngeal bulb slightly elongated (48.0 X 41.4) with two rod-shaped macroplacoids [length configuration 1 (longest) - 2 (shortest)]. Microplacoid and septulum absent. First macroplacoid with distinct central constriction, 9.8 [22.4] long; second 5.8 [13.2] long and without constriction. Macroplacoid row 19.2 [43.8] long. Claws of the Isohypsibius type, similar in size and shape (Figure 4 C). Primary branches of claws with well-developed accessory points. Lunules present and better developed on external claws. Other cuticular thickenings on legs absent. Claw lengths: leg II: external: basal claw 9.1 [20.8], primary branch 17.4 [39.7], secondary branch 11.6 [26.5]; internal: basal claw 8.2 [18.7], primary branch 14.1 [32.2], secondary branch 12.0 [27.4]; leg III: internal: basal claw 8.4 [19.2], primary branch 14.7 [33.6], secondary branch 10.0 [22.8]. Other claws not measurable. Eggs unknown. Etymology The name ‘‘ tergumrudis ’’ refers to the appearance of the posterior end of the body; tergum 5 back, rudis 5 rough. Type depositories The holotype (USNM 1114531) is deposited in the National Museum of Natural History, Smithsonian Institution, Washington, DC, USA. Differential diagnosis Doryphoribius tergumrudis sp. nov. is most similar to Doryphoribius evelinae (Marcus, 1928) but it differs from this species by: the absence of eyes, the absence of gibbosities and tubercles on the first three pair of legs, the presence of granulation instead of tubercles on legs IV, and by the presence of lunules on claws.	en	Bartels, P. J., Nelson, D. R., Kaczmarek, Ł., Michalczyk, Ł. (2008): Three new species and one new record of the genus Doryphoribius Pilato, 1969 (Tardigrada: Eutardigrada: Hypsibiidae) from the Great Smoky Mountains National Park (Tennessee, USA). Journal of Natural History 42 (41 - 42): 2595-2608, DOI: 10.1080/00222930802357343, URL: http://dx.doi.org/10.1080/00222930802357343
4E7587893A7FFFDB87308160FC987D66.taxon	description	Ecological comments All five species of Doryphoribius in our collection [D. smokiensis (Bartels et al. 2007), D. longistipes sp. nov., D. minimus sp. nov., D. tergumrudis sp. nov. and D. doryphorus] appear to be rare and generally restricted to the Cades Cove region of the GSMNP (see Figure 5). The current database for the GSMNP tardigrade collection now includes 10,702 specimens identified to species from over 400 samples throughout the park. Collectively, there are only 29 specimens of Doryphoribius. Three specimens of Doryphoribius smokiensis (Bartels et al., 2007) were found in soil and leaf litter in the dolomitic caves of Cades Cove, while one specimen was found in forest soil and leaf litter outside the limestone-based area of the park, 10 km from the caves (Tremont ATBI plot, see Figure 5). Three specimens of D. longistipes sp. nov. were found in periphyton (but not sediment) from Abram’s Creek just upstream from the limestone-dominated section of Cades Cove. Seven additional specimens of D. longistipes were found in soil and leaf litter from the entrance to Gregory’s Cave. Five specimens of D. minimus sp. nov. were collected from lichens on the external walls of Gregory’s and Bull Caves. Only one specimen of D. tergumrudis sp. nov. has been found in the entire ATBI collection, and it was collected in the periphyton from a limestone-based section of Abram’s Creek. Six specimens of Doryphoribius doryphorus were also found exclusively in periphyton samples from the limestone-based section of Abram’s Creek.	en	Bartels, P. J., Nelson, D. R., Kaczmarek, Ł., Michalczyk, Ł. (2008): Three new species and one new record of the genus Doryphoribius Pilato, 1969 (Tardigrada: Eutardigrada: Hypsibiidae) from the Great Smoky Mountains National Park (Tennessee, USA). Journal of Natural History 42 (41 - 42): 2595-2608, DOI: 10.1080/00222930802357343, URL: http://dx.doi.org/10.1080/00222930802357343
