taxonID	type	description	language	source
03BC87B5FFD7FFFEFF23FC68FD3C60EF.taxon	materials_examined	Holotype. A sagittally-sectioned specimen (EMLC-UP 160) deposited in the Electron Microscopy Laboratory Collection of the Dipartimento di Biologia, Unità di Etologia (Università di Pisa). Type locality. Italy, Tuscany, Ligurian Sea: loc. Partaccia (Marina di Massa, Massa) (44 ° 01 ’ 24 ’’ N, 10 ° 04 ’ 16 ’’ E). Surf-zone characterized by fine sand. Coll. September 2008. Additional material. At least 30 specimens were studied in vivo, including drawings and photographs, all from the type locality. Paratypes (EMLC-UP 160 - A, B, C, D): four specimens from the type locality, sagittally sectioned. Three whole mounts (EMLC-UP 160 - E, F) from the type locality.	en	Meini, Gianluca (2015): Two new Otoplanid species (Platyhelminthes: Rhabditophora: Proseriata) of the genera Orthoplana Steinböck, 1932 and Postbursoplana Ax, 1956 from the Tuscan coast (Italy). Zootaxa 3947 (3): 425-439, DOI: 10.11646/zootaxa.3947.3.9
03BC87B5FFD7FFFEFF23FC68FD3C60EF.taxon	description	Description. The length of sexually mature animals, alive and in extension, reaches 1.4 – 1.6 mm. The body is fusiform, dorsally convex, ventrally flat, colourless and transparent (Figs 1, 2). The anterior end is characterized by a ‘ Köpfchen’ (Ax 1956) or cephalic swelling with numerous tactile hairs (th) and followed by two lateral couples of ‘ Tastborsten’ (Ax 1956) or tactile bristles (tb) retractable into the respective wide pockets (Figs 1, 2, 3, 4). The anterior pair of these bristles is thinner and shorter than the posterior pair, which is characterized by a greater length and thickness. The rhabdites (r), a variety of secretions in epidermal cells or in sub-epidermal glands, are present grouped in longitudinal lines along the body areas without cilia, with the exception of the anterior end, where they are scattered with a low concentration (Fig. 1). The anterior restricted intestine protuberance, called cephalic intestine (ci), with the statocyst (sta) and the ovoid brain (b) are located behind the cephalic swelling. The ovoid brain (b) is lengthened and provided with two - three pairs of lateral rounded protuberances (Figs 1, 2, 3, 4). The testes (te), situated behind the brain and anterior to the pharynx, consist of two close series of medium-size follicles arranged along the longitudinal axis, 11 – 13 per side, in a single line. Two longitudinal rows of small vitellaries (vi) stretch externally to the testes from just behind the brain to the penis papilla opening, uniformly distributed in single units (Figs 1, 2, 3). Two globoid germaries (ge), at some distance from each other, lie in front of the pharynx, posterior to the last testis follicles; they have small dimensions and contain numerous egg cells (Fig. 1). The pharynx (ph), situated in the rear half of the body, exhibits the so-called bell-shaped organization or ‘ Glöckchen’ (Ax, 1956), typical of the genus. The sacciform intestine (i) is a caecum at both ends and the anterior restricted protuberance or cephalic intestine (ci) is constituted by turgid cells (Fig. 1). The accessory male pore (amp) is clearly visible laterally to the penis papilla and can be observed in living and semi-squashed specimens (Figs 1, 7). The caudal end appears sharpened and narrow, provided with numerous adhesive papillae (ap) (Figs 1, 2, 5). In the post-pharyngeal zone, the sacciform vesicula seminalis (vs) is connected distally with a well developed vesicula granulorum (vg). The male sclerotic apparatus (s) consists of a median stylet and 19 spines of variable shape and length (Figs 8, 9, 10): • a “ Trichterrohr ” (T) (according to Ax, 1956) or median stylet, 48 – 49 Μm long, centrally located in the sclerotized complex, club-shaped with pointed tip at both ends; • 1 pair of straight spines, 44 Μm long, arranged at both sides of the median stylet, with a very slight subterminal prominence and a pointed tip. • 17 spines, placed on both sides of the previous ones, practically straight, with pointed tips weakly bent outwards and small subterminal cuneiform prominence. Their length becomes progressively reduced starting from a maximum of 40 Μm, to a minimum of 17 Μm. The proximal end is narrow and blunt.	en	Meini, Gianluca (2015): Two new Otoplanid species (Platyhelminthes: Rhabditophora: Proseriata) of the genera Orthoplana Steinböck, 1932 and Postbursoplana Ax, 1956 from the Tuscan coast (Italy). Zootaxa 3947 (3): 425-439, DOI: 10.11646/zootaxa.3947.3.9
03BC87B5FFD7FFFEFF23FC68FD3C60EF.taxon	discussion	Remarks. Orthoplana lunae sp. nov., is of a similar body length to that observed in O. borealis (1.5 mm), shorter than in O. bregazzii (4 – 5 mm), O. mediterranea (3 – 5 mm) and O. sewardensis (1.5 – 3 mm), and larger than in O. kohni (1.2 – 1.4 mm). Starting from the upper end of the testis rows, the body of the new species shows a constant width until the level of germaries, located in front of the pharynx in the initial part of the caudal body half, as in O. sewardensis and O. mediterranea. The shape of the small cephalic swelling differs from previously described species of the genus: in O. lunae the ‘ Köpfchen’ is ovoid and slightly rounded, while in the other species of the genus the small head is clearly flattened. The distribution and the conspicuous size of the tactile bristles correspond to that described in O. mediterranea and O. kohni, while in O. sewardensis only one pair is visible on the sides of the cephalic swelling. Unlike any other species of the genus, the brain of the new species shows lateral rounded protuberances. The yolk follicle path, arranged in a single longitudinal row from the anterior end to the penis papilla opening on each side of the body, as well as the medium-small follicle dimensions, are similar to that observed in O. mediterranea. In O. sewardensis, the vitellaries are generally similar to those of the new species, but they are different in their distribution and the presence of gaps between every single follicle. The localization of the testes is generally comparable to that observed in two of the species of the genus, but there is a dissimilarity in their distribution: in O. mediterranea there are many more follicles, that reach the level of the upper vitellaries; they are smaller and have gaps between the follicles. In O. sewardensis, the testes have larger dimensions and the presence of gaps between each follicle; which are rounded and few in number. The position of the paired germaries is similar to that observed in O. mediterranea and O. sewardensis, although their dimensions are smaller. In O. kohni, the germaries are much more anterior than the pharynx, and they have a larger dimensions. The sacciform intestine with cephalic protuberance, the pharynx, the position of vesicula seminalis, vesicula granulorum and penis papilla coincide with the other species of the genus. The spines of the sclerotic apparatus of the new species show a different organization compared to that of all the other species described in this genus. They are constituted by a median stylet pointed at both ends, 48 – 49 Μm long, encircled by 19 bristles, with variable shape and length (17 – 44 Μm). In O. mediterranea, the median stylet has a relatively similar but variable length (49 – 85 Μm) and is funnel-shaped, with a sharp narrowing toward the distal pointed end. The new species O. lunae, O. mediterranea and O. kohni have a tube-shaped median stylet with a continuous, uninterrupted wall. O. sewardensis and O. bregazzii are particularly similar regarding their sclerotic apparatus as both species have a groove-shaped stylet. In O. lunae and O. mediterranea, the spines are arranged around the median stylet to form a crown, similar in shape and numbers of spines in each species. In fact, in the former species there are 19 spines, while in the latter 17 – 22 spines are present. They show an elongated and thin body, with a sub-terminal prominence and a bent tip. The different peculiarities between the spines in both species are in their length, 55 – 65 Μm in O. mediterranea and 18 – 44 Μm in O. lunae, and also in their peculiar structures of the distal ends: O. mediterranea has a sub-terminal cuneiform protrusion well distinguishable with an outwards small tip, and the distal extremity is sharply curved to form a hooked tip; the new species displays a poorly developed sub-terminal cuneiform prominence, and the distal end of each spine appears with straight tip upward. On the base of the data presented, O. lunae differs from the previously described species in body dimensions, size and distribution of testes and vitellaries, and, above all, the characteristics of the sclerotic apparatus. The new species has many homologies with O. mediterranea, but it constitutes a valid new otoplanid species.	en	Meini, Gianluca (2015): Two new Otoplanid species (Platyhelminthes: Rhabditophora: Proseriata) of the genera Orthoplana Steinböck, 1932 and Postbursoplana Ax, 1956 from the Tuscan coast (Italy). Zootaxa 3947 (3): 425-439, DOI: 10.11646/zootaxa.3947.3.9
03BC87B5FFD7FFFEFF23FC68FD3C60EF.taxon	etymology	Etymology. The name lunae refers to the type locality where this species has been collected. The site of sampling is on the coast of the territory called Lunigiana, corresponding to the basin of the Magra River located between Liguria, Emilia-Romagna and Tuscany (Ligurian Sea, Italy), and lunae means “ coming from the territory of the ancient Etruscan town of Luna ”. Habitat. Surf zone.	en	Meini, Gianluca (2015): Two new Otoplanid species (Platyhelminthes: Rhabditophora: Proseriata) of the genera Orthoplana Steinböck, 1932 and Postbursoplana Ax, 1956 from the Tuscan coast (Italy). Zootaxa 3947 (3): 425-439, DOI: 10.11646/zootaxa.3947.3.9
03BC87B5FFD7FFFEFF23FC68FD3C60EF.taxon	distribution	Distribution. Known only from type locality.	en	Meini, Gianluca (2015): Two new Otoplanid species (Platyhelminthes: Rhabditophora: Proseriata) of the genera Orthoplana Steinböck, 1932 and Postbursoplana Ax, 1956 from the Tuscan coast (Italy). Zootaxa 3947 (3): 425-439, DOI: 10.11646/zootaxa.3947.3.9
03BC87B5FFDDFFFBFF23FBC6FD3C628A.taxon	materials_examined	Holotype. A sagittally-sectioned specimen (EMLC-UP 161) deposited in the Electron Microscopy Laboratory Collection of the Dipartimento di Biologia, Unità di Etologia (Università di Pisa). Type locality. Italy, Tuscany, Ligurian Sea: loc. Marina di Donoratico (Livorno), (43 ° 09 ’ 14 ’’ N, 10 ° 32 ’ 22 ’’ E). Otoplanen-Zone characterized by medium-fine sand. Coll. April 2007. Additional material. At least 35 specimens were studied in vivo, including drawings and photographs, all from the type locality. Paratypes (EMLC-UP 161 - A, B, C, D, E): three specimens from the type locality, sagittally sectioned. Two whole mounts (EMLC-UP 161 - F, G) from the type locality.	en	Meini, Gianluca (2015): Two new Otoplanid species (Platyhelminthes: Rhabditophora: Proseriata) of the genera Orthoplana Steinböck, 1932 and Postbursoplana Ax, 1956 from the Tuscan coast (Italy). Zootaxa 3947 (3): 425-439, DOI: 10.11646/zootaxa.3947.3.9
03BC87B5FFDDFFFBFF23FBC6FD3C628A.taxon	description	Description. The sexually mature animals are 1.6 – 1.8 mm in body length (Figs 11, 12). Anterior tip characterized by a cephalic elliptic swelling, provided with numerous tactile hairs (th) and two lateral couples of prominent tactile bristles (tb), retractable into the respective wide pockets (Figs 11, 12, 13). The anterior tactile bristles are relatively conspicuous, while the posterior ones are longer and more robust. Tactile hairs are present on the lateral body sides. Behind the cephalic swelling are present the statocyst (sta) and the ovoidal brain (b). The testes (te), located anterior to the pharynx, consist of two series of follicles stretched along the longitudinal axis. There are 8 – 10 per side, generally single, rarely coupled and of medium-large size (Figs 11, 12, 13). Two germaries (ge) of medium-large dimensions, at some distance from each other, are present in front of the pharynx (Fig. 11), posterior to the last testis follicles. They are globoid and contain numerous egg cells. Behind the ovaries two rows of medium – small vitellaries (vi), in single line and laterally placed, and adjacent to the penis papilla opening (Figs 11, 12). The pharynx (ph), perpendicularly situated in the body center, shows the so-called collar-shaped organization (‘ kragenförmig’, Ax, 1956), typical of the genus. The sacciform intestine (i) is a caecum at both extremities (Figs 11, 12). The caudal end is characterized by a narrow and blunt plate provided with a few finger-shaped adhesive papillae (ap) of conspicuous size. These bi-glandular structures are also present in the ventro-lateral epidermis along the body length (Figs 11, 12, 14, 15). The male copulatory organ presents a sclerotic apparatus (s) with 10 spines of variable shape and length (Figs 15, 16, 17, 18, 19): • 1 pair (a), 55 Μm long, placed in the center of the sclerotic complex with a straight body and pointed tip bent outwards; • 1 pair (b), 46 – 48 Μm long, arranged laterally with respect to the previous one, with a small cuneiform subterminal prominence and an outwardly bent pointed tip; • 1 pair (c), 46 Μm long, external to the previous pair (b), with a straight pointed tip. These spines are characterized by the presence of a large cuneiform sub-terminal prominence on the outward side; • 1 pair (d), 52 – 55 Μm long, situated externally with respect to the central spine complex, internally curved with pointed and thin distal end. These spines present a small sub-terminal cuneiform prominence on the concave side; • 1 pair (e), 68 – 70 Μm long, the outermost one placed on both sides of the previous pair (d), with pointed distal tip and sub-terminal cuneiform prominence on the concave side. Similarly to the former couple, these spines have a proximal extremity bulb-shaped.	en	Meini, Gianluca (2015): Two new Otoplanid species (Platyhelminthes: Rhabditophora: Proseriata) of the genera Orthoplana Steinböck, 1932 and Postbursoplana Ax, 1956 from the Tuscan coast (Italy). Zootaxa 3947 (3): 425-439, DOI: 10.11646/zootaxa.3947.3.9
03BC87B5FFDDFFFBFF23FBC6FD3C628A.taxon	discussion	Remarks. The new species shows a body length similar but slightly larger than P. tyrrhenica (1.5 - 1.6 mm), while all the remaining species of the genus have a smaller length (between 0.4 and 1.4 mm). The organization of the cephalic bulge is similar to that observed in P. propontica, while it is much less similar to that found in P. fibulata, P. minima and P. pontica. The distribution of the testes is typical of the genus, but their path and number are similar to those described in P. fibulata. Moreover, the dimensions of the testicular follicles in the new species are similar to those present in P. fibulata, P. pontica, P. tyrrhenica and P. parafibulata. The paired germaries, located frontally to the pharynx, show a localization similar to those observed in P. fibulata and P. parafibulata, while their sizes are comparable to those found in P. pontica, P. propontica and P. parafibulata. The position of the vitellaria, arranged in a single longitudinal row on both body sides, is typical of the genus; however, their arrangement is similar to that observed in P. fibulata, P. propontica and P. tyrrhenica. In P. donoraticensis, the medium-small size of vitellaries, which increase towards the penis papilla, are similar to those found in P. pontica and P. propontica. The pharynx displays the typical characteristics observed in all the genera of the subfamily, although its location in the organism is more central, similarly to that described in P. fibulata and in P. parafibulata. The intestine is similar to that of all the genus species, sacciform and without a cephalic intestine. The locations of vesicula seminalis, vesicula granulorum and penis papilla appear to coincide with those of the other species. The spines of the male copulatory organ of the new species display a different organization from that of all the other species. The overall number of spines is 10, as in P. fibulata, P. macromystax and P. parafibulata, while all the other Postbursoplana species have only eight spines. In P. donoraticensis, the central pair (a) shows a pointed distal end with a short curvature, similar to that present in P. fibulata, P. macromystax, P. pontica and P. propontica. These two spines are larger (55 Μm) than those found in previously described species, where their length varies from 29 to 48 Μm, with the exception of P. parafibulata in which these spines are longer (61 – 62 Μm). The pair of spines (b), at both sides of this central longer pair, is very similar to the equivalent spines of P. fibulata. The length of these spines in P. donoraticensis (46 – 48 Μm) exceeds that of P. minima (24 Μm) and that of P. tyrrhenica (26 – 27 Μm), and is lower than the equivalent spines present in P. parafibulata (52 – 53 Μm). The third spine pair (c) presents a shape similar to those observed in P. fibulata, P. minima, P. pontica and P. propontica, in which are obvious slight differences in the distal part with a sub-terminal cuneiform prominence less wide. In the new species, the length (46 Μm) of these spines is greater than that measured in P. fibulata (43 Μm), P. minima (35 Μm), P. pontica (26 – 27 Μm) and P. propontica (38 Μm), with the exception of P. parafibulata that instead shows a greater length (52 – 53 Μm) of these spines. The pointed tip of these spines reminds that observed in P. propontica, while the cuneiform sub-terminal prominence is similar to those described in P. macromystax, P. propontica, P. t y r rh e ni c a and P. parafibulata. In P. donoraticensis, the spines of the pair d (52 – 55 Μm long) are similar to the homologous spines present in P. fibulata (56 Μm) and P. parafibulata (52 – 53 Μm). In all other species the equivalent spines have a shorter length (between 30 – 32 Μm and 44 – 48 Μm). The length (68 – 70 Μm) of the most external pair of spines (e), exceeds that observed in the other species (40 – 60 Μm), with the exception of P. parafibulata (70 – 73 Μm). On the base of the data presented, P. donoraticensis differs from the previously described species in body dimensions and, above all, the characteristics of the sclerotic apparatus. The new species has a close relationship with P. fibulata, but represents a new species.	en	Meini, Gianluca (2015): Two new Otoplanid species (Platyhelminthes: Rhabditophora: Proseriata) of the genera Orthoplana Steinböck, 1932 and Postbursoplana Ax, 1956 from the Tuscan coast (Italy). Zootaxa 3947 (3): 425-439, DOI: 10.11646/zootaxa.3947.3.9
03BC87B5FFDDFFFBFF23FBC6FD3C628A.taxon	etymology	Etymology: The name donoraticensis refers to the type locality where this species has been collected. The site of sampling is very close to the village of Marina di Donoratico (Tuscany, Ligurian Sea, Italy), and donoraticensis means “ coming from Donoratico ”. Habitat. Surf zone.	en	Meini, Gianluca (2015): Two new Otoplanid species (Platyhelminthes: Rhabditophora: Proseriata) of the genera Orthoplana Steinböck, 1932 and Postbursoplana Ax, 1956 from the Tuscan coast (Italy). Zootaxa 3947 (3): 425-439, DOI: 10.11646/zootaxa.3947.3.9
03BC87B5FFDDFFFBFF23FBC6FD3C628A.taxon	distribution	Distribution. Known only from type locality.	en	Meini, Gianluca (2015): Two new Otoplanid species (Platyhelminthes: Rhabditophora: Proseriata) of the genera Orthoplana Steinböck, 1932 and Postbursoplana Ax, 1956 from the Tuscan coast (Italy). Zootaxa 3947 (3): 425-439, DOI: 10.11646/zootaxa.3947.3.9
