taxonID	type	description	language	source
038B832DFF809060FF16CB34AE8AADE2.taxon	description	As the aim of our project was to give access to the partial 12 S ribosomal RNA sequence (i. e, the marker most often used for e DNA studies) and the partial COI gene commonly used for barcoding, a part of the mitochondrial genome (called in this study ‘ Mt- 1 ’ for ‘ the first part of the mitogenome’ starting from the middle of the 12 S to the tRNA-Ser after the COI gene) was amplified following the protocol established by Hinsinger et al. (2015) using the specific primers 12 S-L 1091 R 5 ’ AAACTGGGATTAGA- TACCCCACTAT 3 ’ (Kocher et al., 1989) and MtH 7061 5 ’ GGGTTATGTGGCTGGCTTGAAAC 3 ’ (Hinsinger et al., 2015). The Mt- 1 fragment provides the sequence of 12 S gene [around 500 bp] and the complete COI gene. Ampli- con libraries (Hinsinger et al., 2015) were then sequenced on an Illumina MiSeq sequencer following the manufacturer’s instructions. When the Mt- 1 fragment reconstruction was difficult, a fragment of the COI gene was amplified with the specific fish primers TelF 1 and TelR 1 (Dettaï et al., 2011) and used as a reference. Data processing was done in Geneious v. 11.1.2 (http: // www. geneious. com, Kearse et al., 2012). To reconstruct the Mt- 1 fragment of each individual, we used the mtDNA of the species identified morphologically or of the same genus (personal data or sequences available on GenBank) as a starting reference (i. e., for a Map to reference). For the species that had not been sequenced yet at the time of our project, such as, for example, Bleheratherina pierucciae Aarn and Ivantsoff, 2009, a piece of the COI gene was obtained by Sanger sequencing and then used as a starting reference. The consensus of each Mt- 1 fragment was primarily checked manually (assembly success, coverage assessment, comparison to available COI sequences for the same specimen, BLAST searches; Altschul et al., 1997). Then the consensus sequence was annotated using MitoAnnotator (Iwasaki et al., 2013) and each gene was checked for coding sequences, stop codons, position of the SNPs.	en	Mennesson, Marion I., Charpin, Nicolas, Keith, Philippe (2024): Fish conservation: importance of DNA reference library based on accurately identified specimens. The case of New Caledonian freshwater fish. Cybium 48 (3): 187-193, DOI: 10.26028/cybium/2024-014, URL: http://dx.doi.org/10.5281/zenodo.14681635
