taxonID	type	description	language	source
0387AA009B04B2111056D849FCC9B662.taxon	description	Description The trophozoite is elongated, irregular flat ribbon shaped contains tapering pointed end. Ectosarc is thick and the endosarc is vacuolated. Large, rounded very distinct nucleus is present at the middle portion of the body. Syzygy is present. In the life cycle of the present form two unequal overlapping almost rounded gametocyte have been found. Oocyst is biconical with pointed ends. The trophozoite measures 132.0 - 160.0 (145.0 ± 4.50) x 30.0 - 35.0 (32.2 ± 2.50) µm. Nucleus measuring 15.5 - 20.0 (17.2 ± 1.5) µm in diameter. Gametocyst measures 60.0 - 70.0.5 (65.0 ± 2.50) µm. Oocyst measures 9.0 - 12.5 (11.0 ± 1.5) x 6.5 - 7.5 (7.0 ± 0.96) µm. Taxonomic Summary Type host: Eutyphoeus orientalis Site of infection: Seminal vesicles Distribution: Bakkhali (Sundarban) region, South 24 parganas (22.1352 ºN, 88.4016 ºE), West Bengal, India Prevalence: 10 out of 25 host earthworms (40 %) were infested with the parasite.	en	Sarkar, Sutapa, Kundu, Beauty, Bandyopadhyay, Probir K. (2019): Taxonomical studies of four new Aseptate Gregarine parasites belonging to the Genus Monocystis Stein, 1848 (Protozoa: Apicomplexa: Sporozoa) from an Oligochaete Host, Eutyphoeus orientalis (Annelida: Oligochaeta) of West Bengal, India. Records of the Zoological Survey of India 119 (4), DOI: 10.26515/rzsi/v119/i4/2019/142223
0387AA009B04B2111056D849FCC9B662.taxon	materials_examined	Material deposited Parasitology Laboratory, Department of Zoology, University of Kalyani, Kalyani- 741235, Nadia, West Bengal, India. Paratype: The paratype has been deposited in the Harold WManter, Laboratory Parasitology, Systematic Research Collection University of Nebraska State Museum W 436, Nebraska Hall, Lincoin, Nebraska 68588 - 0514 USA 4 (Accession no. P- 2014 - 002: Reg. no. HWML 49895). Etymology: The species name have been given after the name of renowned parasitologist Prof. Csaba Szekely of Institute for Veterinary Medical Research, Centre for Agriculture Research, Hungarian Academy of Sciences, Budapest Hungaria Krt. 21 for his outstanding contribution in the field of fish parasitology. Holotype: PR / MC / 34 / 12 has been deposited at the Remark The present species under discussion compares with Monocytsis apporectodae and Monocystis elongatum. The species under discussion has elongated, irregular, flat and ribbon like trophozoite, appears gradually tapering pointed end which is flame like in appearance. Whereas, it is elongated, irregular, flat like having both side pointed ends in M. apporectodae and in M. elongatum it is elongated and curved. Large and rounded nucleus is present in the present form. But in M. apporectodae, it is ovoidal contains densely packed karyosome and in M. elongatum nucleus is rounded to ovoid. Ectosarc is thick in the present specis while, in M. apporectodae, it is thin and in M. elongatum it is smooth. The present form has vacuolated endoplasm whereas in earlier described species endoplasm is densely granulated. The syzygy stage is found in the life cycle of both species. Moreover, the size and shape of the gametocysts and oocysts of the current form are also different from the previously described	en	Sarkar, Sutapa, Kundu, Beauty, Bandyopadhyay, Probir K. (2019): Taxonomical studies of four new Aseptate Gregarine parasites belonging to the Genus Monocystis Stein, 1848 (Protozoa: Apicomplexa: Sporozoa) from an Oligochaete Host, Eutyphoeus orientalis (Annelida: Oligochaeta) of West Bengal, India. Records of the Zoological Survey of India 119 (4), DOI: 10.26515/rzsi/v119/i4/2019/142223
0387AA009B0CB2191056DEECFBB2B423.taxon	description	Description In Monocystis eutyphae sp. nov. gamonts are fusiform, anterior portion is wider than the posterior end. The anterior end contains mucron. Nucleus is rounded, situated in the middle portion of the body. The pellicle is thin. Presence of highly granulated endosarc contains paraglycogen granules. Gametocyst ovoid with two unequal gametocytes. Oocysts are biconical. Gamont measures 100.7 - 107.8 (104.83 ± 2.02) µm in length and width of the anterior part of gamont is 51.93 - 59.67 (55.68 ± 3.13) µm and Posterior part is 20.99 - 23.86 (22.69 ± 1.08) µm. Diameter of nucleus is 10.5 - 11.0 (9.0 ± 1.05). Gametocyst measures 89.50 - 96.13 (93.04 ± 2.81) µm in length and 67.40 - 78.45 (72.37 ± 4.35) µm in width. Length of the oocyst measuring 13.25 - 15.47 (14.30 ± 0.91) µm and width measuring 9.94 - 13.26 (11.70 ± 1.40) µm. Taxonomic Summary Type host: Eutyphoeus orientalis. Site of infection: Seminal vesicles. Type locality: Ashoknagar, North 24 Parganas (23 º 15 ’ 2 ’’ N- 89 º 5 ’ E), West Bengal, India. Prevalence: 20 out of 40 host earthworms (50 %) were infested with the parasite.	en	Sarkar, Sutapa, Kundu, Beauty, Bandyopadhyay, Probir K. (2019): Taxonomical studies of four new Aseptate Gregarine parasites belonging to the Genus Monocystis Stein, 1848 (Protozoa: Apicomplexa: Sporozoa) from an Oligochaete Host, Eutyphoeus orientalis (Annelida: Oligochaeta) of West Bengal, India. Records of the Zoological Survey of India 119 (4), DOI: 10.26515/rzsi/v119/i4/2019/142223
0387AA009B0CB2191056DEECFBB2B423.taxon	materials_examined	Material deposited Holotype: PR / MC / 01 / 12 has been deposited at the Parasitology Laboratory, Department of Zoology, University of Kalyani 741235, and West Bengal, India. Paratype: The paratype has been deposited in the Harold W Manter, Laboratory of Parasitology, Systematic Research Collections, University of Nebraska State Museum, W 436, Nebraska Hall, Lincoln, Nebraska 68588 - 0514 USA, (Accession no. P- 2014 - 002; Reg. no. HWML 49901). Etymology: The species name has been derived from the generic name of the host specimen, Eutyphoeus orientalis. of Eutyphoeus orientalis	en	Sarkar, Sutapa, Kundu, Beauty, Bandyopadhyay, Probir K. (2019): Taxonomical studies of four new Aseptate Gregarine parasites belonging to the Genus Monocystis Stein, 1848 (Protozoa: Apicomplexa: Sporozoa) from an Oligochaete Host, Eutyphoeus orientalis (Annelida: Oligochaeta) of West Bengal, India. Records of the Zoological Survey of India 119 (4), DOI: 10.26515/rzsi/v119/i4/2019/142223
0387AA009B0CB2191056DEECFBB2B423.taxon	discussion	Remark The species under discussion shows similarities and dissimilarities with Monocystis lalbagensis and Monocystis nadiensis respectively. The present species differs from M. Lalbagensis relating its shape and size of the body. Trophozoites are cone shaped in M. lalbagensis, while they are balloon shaped in M. nadiensis and fusiform in M eutyphae sp. nov. In M. lalbagensis vacuoles excavate the endosarc whereas, in M. nadiensis, cytoplasm is densely granulated as like as that of present species. The anterior end of present species is wider than the posterior end which looks like blunt shaped. A big ovoidal distinct mucron is present in the anterior portion. The nucleus is rounded, present near the mucron at anterior portion of the body. Pellicle is thin. In the present form syzygy stage is present in the life cycle, while this stage is absent in the earlier described species. The position, shape and size of the nucleus, gametocyst and oocyst, host and the geographic location of the present species differ from the earlier described species (Table 1). Taking into consideration all the differences with the closely related species, the present species seems to be new to science. Hence the name Monocystis eutyphae sp. nov. is being proposed here.	en	Sarkar, Sutapa, Kundu, Beauty, Bandyopadhyay, Probir K. (2019): Taxonomical studies of four new Aseptate Gregarine parasites belonging to the Genus Monocystis Stein, 1848 (Protozoa: Apicomplexa: Sporozoa) from an Oligochaete Host, Eutyphoeus orientalis (Annelida: Oligochaeta) of West Bengal, India. Records of the Zoological Survey of India 119 (4), DOI: 10.26515/rzsi/v119/i4/2019/142223
0387AA009B09B21D13B5DA9DFF3CB252.taxon	description	Description (31.50 ± 2.50) µm. Oocyst measures 10.55 - 11.00 (10.77 ± 0.46) µm x 7.00 - 8.50 (6.0 ± 0.50) µm. In Monocystis indicus sp. nov. gamonts are oval shaped. Rounded nucleus is present at middle region of the body. Presence of smooth, thin and irregular shaped ectosarc. It contains highly granulated endosarc. At the anterior end of the body spherical mucron is present. Syzygy stage present in the life cycle. Gametocyst contains two unequal gametocytes and almost rounded in shape. Oocyst are biconical. Gamont measures 89.61 - 111.6 (102.95 ± 9.69) µm. x 59.29 - 70.72 (66.20 ± 4.33) µm. Nucleus measures 14.30 - 20.4 (18.50 ± 2.00) µm in diameter. Gametocyst measures 90.25 - 102.50 (39.25 ± 5.55) µm in diameter. Large gametocyte measures 66.50 - 75.50 (70.90 ± 4.50) µm x 35.80 - 45.50 (40.50 ± 3.25) µm and small gametocyte measures 60.40 - 65.20 (64.54 ± 3.54) µm x 25.50 - 35.28 Taxonomic Summary Type host: Eutyphoeus orientalis Site of infection: Seminal vesicles Type locality: Ashoknagar, North 24 Parganas (23 ° 15 ' 2 " N- 89 ° 5 ' E), West Bengal, India. Prevalence: 15 out of 40 host earthworms (37.5 %) were infested with the parasite.	en	Sarkar, Sutapa, Kundu, Beauty, Bandyopadhyay, Probir K. (2019): Taxonomical studies of four new Aseptate Gregarine parasites belonging to the Genus Monocystis Stein, 1848 (Protozoa: Apicomplexa: Sporozoa) from an Oligochaete Host, Eutyphoeus orientalis (Annelida: Oligochaeta) of West Bengal, India. Records of the Zoological Survey of India 119 (4), DOI: 10.26515/rzsi/v119/i4/2019/142223
0387AA009B09B21D13B5DA9DFF3CB252.taxon	materials_examined	Material deposited Holotype: PR / MC / 03 / 12 has been deposited in the Parasitology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, West Bengal, India. Paratype: Systematic Research Collection, University of Nebraska State Museum, W 436. Nebraska Hall. Lincoin, Nebraska 68588 - 0514 USA (Acession no. P- 2014 - 002; Reg. no. HWML 49896). Etymology: The species names have been given after the name of the nation India. orientalis	en	Sarkar, Sutapa, Kundu, Beauty, Bandyopadhyay, Probir K. (2019): Taxonomical studies of four new Aseptate Gregarine parasites belonging to the Genus Monocystis Stein, 1848 (Protozoa: Apicomplexa: Sporozoa) from an Oligochaete Host, Eutyphoeus orientalis (Annelida: Oligochaeta) of West Bengal, India. Records of the Zoological Survey of India 119 (4), DOI: 10.26515/rzsi/v119/i4/2019/142223
0387AA009B09B21D13B5DA9DFF3CB252.taxon	discussion	Remark While comparing the present species with earlier described species Monocystis ayeshae and M. arabindae, it appears that the gamont of M. ayeshae is oval shaped bearing swolen middle part as that of the present species and in Monocystis arabindae the gamont is solitary, cylindrical curved due to presence of constriction at the lateral surface. In present species, mucron is prominent while in M. ayeshae and M. arabindae it is absent. In M. ayeshae, ectosarc is smooth, thin and granulated endosarc devoid of any spots. Nucleus is large and oval shaped. Whereas, ectosarc is smooth and thin in present species and highly granulated endosarc contains large granules. Nucleus is rounded and present at the middle portion of the body. In M. arabindae ectosarc is granulated and vaculated endosarc, nucleus rounded. The gametocyst of M. ayeshae is almost rounded contains unequal gametocytes as that of present species. While in M. arabindae gametocyst is ellipsoidal. In M. ayeshae, oocyst is navicular with pointed ends while it is biconical in M. arabindae as that of present form. Syzygy stage found in present form. While it is not present in M. ayeshae and M. arabindae. Moreover, M. ayeshae and M. arabindae have been reported from Metaphire posthuma of Satkhira, Bangladesh and Eutyphoeus incommodus from West Midnapore, West Bengal, India respectively but the present species have been described from the Eutypohoeus orientalis collected from Ashoknagar, West Bengal, India (Table 2). Considering all the differences with the closely related species the present species seems to be new to science. Hence the name Monocystis indicus sp. nov. is being proposed.	en	Sarkar, Sutapa, Kundu, Beauty, Bandyopadhyay, Probir K. (2019): Taxonomical studies of four new Aseptate Gregarine parasites belonging to the Genus Monocystis Stein, 1848 (Protozoa: Apicomplexa: Sporozoa) from an Oligochaete Host, Eutyphoeus orientalis (Annelida: Oligochaeta) of West Bengal, India. Records of the Zoological Survey of India 119 (4), DOI: 10.26515/rzsi/v119/i4/2019/142223
0387AA009B0AB2131390DB39FA6CB2E2.taxon	description	Description Gamonts are elongated, flat and oval shaped. One end rounded and other cone shaped. Presence of smooth and thick ectosarc. Endosarc is vacuolated. Presence of distinct rounded nucleus at the conical portion of the gamonts. Gametocysts are rounded contains two unequal gametocytes. Oocysts are biconical having pointed ends. The gamont measures 96.55 - 125.00 (15.25 ± 8.25) x 50.28 - 62.50 (59.22 ± 5.00) µm. Nucleus measuring 12.35 - 14.50 (13.75 ± 1.00) µm in diameter. Gametocyst measures 70.00 - 75.00 (72.00 ± 4.32) µm in diameter. Oocyst measures 15.17 - 18.17 (16.70 ± 1.35) in length 9.75 - 11.25 (10.00 ± 1.00) µm in width. Taxonomic Summary Type host: Eutyphoeus orientalis Site of infection: Seminal vesicles Distribution: Ashoknagar, North 24 Parganas (23 ° 15 ' 2 " N- 89 ° 5 ' E), West Bengal, India. Prevalence: 10 out of 35 host earthworms (28.57 %) were infested with the parasite.	en	Sarkar, Sutapa, Kundu, Beauty, Bandyopadhyay, Probir K. (2019): Taxonomical studies of four new Aseptate Gregarine parasites belonging to the Genus Monocystis Stein, 1848 (Protozoa: Apicomplexa: Sporozoa) from an Oligochaete Host, Eutyphoeus orientalis (Annelida: Oligochaeta) of West Bengal, India. Records of the Zoological Survey of India 119 (4), DOI: 10.26515/rzsi/v119/i4/2019/142223
0387AA009B0AB2131390DB39FA6CB2E2.taxon	materials_examined	Material deposited Holotype: PR / MC / 05 / 12 has been deposited at the Parasitology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, West Bengal, India. Paratype: The paratype has been deposited in the Harold W Manter, Laboratory of Parasitology, Systematic Research Collections, University of Nebraska State Museum, W 436, Nebraska Hall, Lincoln, Nebraska 68588 - 0514 USA, (Accession no. P- 2014 - 002; Reg. no. HWML 49896). Etymology: The species name have been given after the name of renowned parasitologist Prof. Hiroshi Sato, Joint Faculty of Veterinary Medicine of Yamaguchi University, 1677 - 1 Yoshida, Yamaguchi, 753 - 8515, Japan for his outstanding contribution in the field of Parasitology.	en	Sarkar, Sutapa, Kundu, Beauty, Bandyopadhyay, Probir K. (2019): Taxonomical studies of four new Aseptate Gregarine parasites belonging to the Genus Monocystis Stein, 1848 (Protozoa: Apicomplexa: Sporozoa) from an Oligochaete Host, Eutyphoeus orientalis (Annelida: Oligochaeta) of West Bengal, India. Records of the Zoological Survey of India 119 (4), DOI: 10.26515/rzsi/v119/i4/2019/142223
0387AA009B0AB2131390DB39FA6CB2E2.taxon	discussion	Remark While compairing, the present species with Monocystis nidata, Monocystis pontodrilli and Monocystis darjeelingensis, it appears that the trophozoite is flat and oval shaped having one rounded and other conical end in the present form. Whereas in M. nidata, it looks like a small cell which possess two blunt ends, but in M. pontodrilli it is elongated having two broadly rounded ends. In M. pontodrilli the trophozoite crescent shaped and mucron is present in one end of the trophozoite. On the contrary, it is also crescent shaped in the present form. Nucleus is rounded and present in conical portion of the present species. But in M. nidata and M. pontodrilli, it is ovoid, centralized and rounded present either at the anteriormiddle or the posterior end respectively. Trophozoite is cylindrical with rounded extremities of one end and slightly wider than other in M. Darjeelingensis. In M. Darjeelingensis, nucleus is spherical in shape and present in the narrow portion. Furthermore, M. darjeelingensis is much larger in all respects than the present form and earlier described other species. The oocyst is navicular in M. nidata and M. darjeelingensis respectively. While, in M. Pontodrilli it is spindle or boat shaped but biconical in the current species. Moreover, M. nidata and M. pontodrilli have been described from Pontodrilus ephippiger of Asia and Indo-China and Pontodrilus barmudensis of South India respectively whereas, M. darjeelingensis and the present species have been reported from Amynthas robusta of Senchal lake of Darjeeling and Eutyphoeus orientalis of Ashoknagar, North 24 Parganas district of West Bengal, India respectively. Taking into account all the differences with the closely related species, the present species seems to be new one. Hence the name Monocystis satoi sp. nov. is given here.	en	Sarkar, Sutapa, Kundu, Beauty, Bandyopadhyay, Probir K. (2019): Taxonomical studies of four new Aseptate Gregarine parasites belonging to the Genus Monocystis Stein, 1848 (Protozoa: Apicomplexa: Sporozoa) from an Oligochaete Host, Eutyphoeus orientalis (Annelida: Oligochaeta) of West Bengal, India. Records of the Zoological Survey of India 119 (4), DOI: 10.26515/rzsi/v119/i4/2019/142223
