identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
0386492CFFFF3F4A9AE5FA342828F872.text	0386492CFFFF3F4A9AE5FA342828F872.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna	<div><p>DNA Extraction, PCR Amplification, and Sequencing</p><p>For molecular characterization, genomic DNA was isolated from fungal mycelia using the Trelief™ Plant Genomic DNA Kit (TSINGKE Biotech, Shanghai, China) following the manufacturer's instructions, with subsequent storage at -20°C for preservation. Five phylogenetic markers were targeted for amplification: ITS, SSU, LSU, TEF 1, and RPB2. PCR amplification was performed in 30 μL reaction volumes containing: 2 μL genomic DNA template, 1 μL each of forward and reverse primers (ITS9mun/ITS4_KYO1 (Egger 1995; Toju et al. 2012) for ITS; LR0R/LR5 (Vilgalys &amp; Hester 1990; Cubeta et al. 1991) for LSU; PNS1/NS41 (Hibbett 1996) for SSU; EF1-983F/EF1-2218R for TEF 1 (Carbone &amp; Kohn 1999; Rehner &amp; Buckley 2005); fRPB2-5F/fRPB2-7cR (Carbone &amp; Kohn 1999; Rehner &amp; Buckley 2005) for RPB2, 11 μL sterile ddH 2 O, and 15 μL 2× Flash PCR Master Mix (containing Taq DNA polymerase, dNTPs, Mg²⁺, and optimized buffer; CoWin Biosciences, Nanjing, China). Thermal cycling parameters included: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 95°C (15 s), annealing at 55°C (25 s), and extension at 72°C (25 s); followed by final extension at 72°C for 5 min. Successful amplification was confirmed through electrophoretic separation on 1% agarose gels. Sanger sequencing was conducted by Tsingke Biological Technology (Beijing, China). The newly generated sequences were deposited in GenBank, and their accession numbers are listed in Table 1.</p></div>	https://treatment.plazi.org/id/0386492CFFFF3F4A9AE5FA342828F872	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Liao, Yue-Chi;Deng, Li-Ping;Chen, Yan-Peng;Liu, Jian-Kui;Maharachchikumbura, Sajeewa S. N.	Liao, Yue-Chi, Deng, Li-Ping, Chen, Yan-Peng, Liu, Jian-Kui, Maharachchikumbura, Sajeewa S. N. (2025): Conioscypha pruni sp. nov. (Conioscyphaceae, Conioscyphales), from sweet cherry (Prunus avium) from Sichuan Province, China. Phytotaxa 716 (2): 117-127, DOI: 10.11646/phytotaxa.716.2.4, URL: https://doi.org/10.11646/phytotaxa.716.2.4
0386492CFFFB3F4D9AE5FF14295AFCF2.text	0386492CFFFB3F4D9AE5FF14295AFCF2.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Conioscypha pruni Y. C. Liao & Maharachch. 2025	<div><p>Conioscypha pruni Y. C. Liao &amp; Maharachch., sp. nov.</p><p>MycoBank: 860245</p><p>Holotype:— HKAS 148727</p><p>Etymology:—The species epithet refers to the host genus where the fungus was collected.</p><p>Figure 2. Conioscypha pruni (HKAS 148727, holotype). a–i Colonies on the natural substrate. a–c Colonies. d conidiogenous cells with attached conidia. e-i Conidia. j Germinating conidium. k, l Colony on PDA from above and below. m–q Sporulation observed on PDA. m, n Conidia and conidiogenous cell. o–q Conidia. Scale bars: d–j, m–q = 10 μm.</p><p>Saprobic on dead branches of sweet cherry trees. Sexual morph: Undetermined. Asexual morph: Hyphomycetous. Colonies on natural substratum, effuse, scattered to gregarious, brown, shiny, superficial. Mycelium partly superficial, composed of septate, branched, subhyaline hyphae. Conidiophores macronematous, often reduced to conidiogenous cells. Conidiogenous cells 2.5–4 μm wide (x̄ = 2.91 μm, n = 10), enteroblastic, with a small cup-like collarette, hyaline, and smooth-walled. Conidia 6–10 × 3.5–5 μm (x̄ = 7.52 × 4.53 μm, n = 30), pleuroacrogenous, aseptate, guttulate, cylindrical, with rounded apices, pale brown when young, dark brown when mature, smooth-walled.</p><p>Culture characteristics: Conidium germinated on PDA within 24 h. Colonies reaching a diameter of about 20 mm after 3 months at 25 °C, colonies from above: irregular margins, coriaceous, dry, pale yellow, distinct black punctiform pigmentation from the reverse. Mycelium hyaline, 1.5–2.5 µm wide. Conidiophores often reduced to conidiogenous cells, Conidiogenous cells hyaline, 2.0–2.5 µm wide, with cup-like collarette. Conidia hyaline to brown, subglobose to ellipsoidal, 6–13 × 3.5–6 μm (x̄ = 8.39 × 4.36 μm, n = 30).</p><p>Material examined: CHINA. Sichuan Province:Chengdu City,Yunhua Mountain, Dujiangyan County, 31°00'74"N, 103°56'19"E, 1535 m, on the decaying branches of sweet cherry, 22 March 2024, Yan Jin, YHS5_1 (HKAS 148727, holotype), ex-type living culture CGMCC 3.29000 = UESTCC 24.0251, HUEST 24.0263, isotype.</p><p>Notes: Phylogenetic analysis based on the combined dataset of ITS, LSU, SSU, RPB 2, and TEF 1 loci revealed that our collection (HUEST 24.0263) forms a monophyletic clade sister to C. minutispora (FIGURE 1). In the NCBI BLASTn search, the closest match to our isolate (UESTCC 24.0251) is C. minutispora . BLASTn analysis revealed 5.2% differences in ITS (436/460 bp, 8 gaps), 5.2% differences in LSU (626/661 bp, without gaps), and 99% sequence identity in SSU (938/940 bp, 2 gaps) between UESTCC 24.0251 and C. minutispora . Morphologically, UESTCC 24.0251 differs from C. minutispora by comparatively shorter conidiogenous cells (2.5–4 μm vs. 4–5 μm) and by narrower conidia (3.5–5 μm vs. 5–6 μm). Conidia of the new species are guttulate, ellipsoidal, contrasting with C. minutispora, which produces conidia with distinctly truncate bases and a central pore. Therefore, based on morphological and phylogenetic evidence, our isolate is proposed as a new species.</p></div>	https://treatment.plazi.org/id/0386492CFFFB3F4D9AE5FF14295AFCF2	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Liao, Yue-Chi;Deng, Li-Ping;Chen, Yan-Peng;Liu, Jian-Kui;Maharachchikumbura, Sajeewa S. N.	Liao, Yue-Chi, Deng, Li-Ping, Chen, Yan-Peng, Liu, Jian-Kui, Maharachchikumbura, Sajeewa S. N. (2025): Conioscypha pruni sp. nov. (Conioscyphaceae, Conioscyphales), from sweet cherry (Prunus avium) from Sichuan Province, China. Phytotaxa 716 (2): 117-127, DOI: 10.11646/phytotaxa.716.2.4, URL: https://doi.org/10.11646/phytotaxa.716.2.4
