taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
03F0E042C54EFF93FF67F9396317FBB7.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/12828490/files/figure.png	https://doi.org/10.5281/zenodo.12828490	Fig. 2. PhyML-phylogeny of the order Siluriformes, including 32 catfish families (117 sequences) of three suborders, Siluroidei, Loricarioidei, and Diplomystoidei based on the complete concatenated nucleotide sequences of all 13 mitochondrial protein coding genes (about 11,408 bp in length) (Table S2). Two sequences of Gonorynchiformes were used as an outgroup. The alignment was performed by MAFFT (Katoh and Standley 2013), curated by BMGE v1.12 (Criscuolo and Gribaldo 2010), the tree was reconstructed in PhyML 3.3 (Guindon et al. 2010) using a maximum likelihood method and 1000 bootstrap resamplings, and the output Newick tree was extracted and visualized using FigTree v1.4.4 (Rambaut 2018). The nodal bootstrap support values (shown at each node) were interpreted from the concurrently constructed tree using the above MAFFT-BMGE alignment by MEGA X (Kumar et al. 2018). The basal nodes of the three suborders (Diplomystoidei, Loricarioidei, and Siluroidei) as well as the two major “Big Asia” and “Big Africa” groups (background highlighted) are shown by arrows. The Pangasius mekongensis, Pangasianodon hypophthalmus, and Pangasius krempfi sequences in this study are indicated by stars and with the associated families’ background highlighted. The taxa were presented with their full names. The abbreviations of the isolates are given in brackets, including the geographical origin or voucher records of each sequenced specimen (where available), which were retrieved from the previous studies (Saitoh et al. 2003; Nakatani et al. 2011; Kappas et al. 2016; Zhang et al. 2021; Schedel et al. 2022). The country of origin or geographical regions where the sample was reported are given in full name, if available. Accession numbers are given at the end of each sequence label. The scale bar represents the number of substitutions per site.	Fig. 2. PhyML-phylogeny of the order Siluriformes, including 32 catfish families (117 sequences) of three suborders, Siluroidei, Loricarioidei, and Diplomystoidei based on the complete concatenated nucleotide sequences of all 13 mitochondrial protein coding genes (about 11,408 bp in length) (Table S2). Two sequences of Gonorynchiformes were used as an outgroup. The alignment was performed by MAFFT (Katoh and Standley 2013), curated by BMGE v1.12 (Criscuolo and Gribaldo 2010), the tree was reconstructed in PhyML 3.3 (Guindon et al. 2010) using a maximum likelihood method and 1000 bootstrap resamplings, and the output Newick tree was extracted and visualized using FigTree v1.4.4 (Rambaut 2018). The nodal bootstrap support values (shown at each node) were interpreted from the concurrently constructed tree using the above MAFFT-BMGE alignment by MEGA X (Kumar et al. 2018). The basal nodes of the three suborders (Diplomystoidei, Loricarioidei, and Siluroidei) as well as the two major “Big Asia” and “Big Africa” groups (background highlighted) are shown by arrows. The Pangasius mekongensis, Pangasianodon hypophthalmus, and Pangasius krempfi sequences in this study are indicated by stars and with the associated families’ background highlighted. The taxa were presented with their full names. The abbreviations of the isolates are given in brackets, including the geographical origin or voucher records of each sequenced specimen (where available), which were retrieved from the previous studies (Saitoh et al. 2003; Nakatani et al. 2011; Kappas et al. 2016; Zhang et al. 2021; Schedel et al. 2022). The country of origin or geographical regions where the sample was reported are given in full name, if available. Accession numbers are given at the end of each sequence label. The scale bar represents the number of substitutions per site.	2023-09-22	Duong, Thuy Yen;Pham, Linh Thi Khanh;Le, Xuyen Thi Kim;Nguyen, Ngoc Tran Thi;Nor, Siti Azizah Mohd;Le, Thanh Hoa		Zenodo	biologists	Duong, Thuy Yen;Pham, Linh Thi Khanh;Le, Xuyen Thi Kim;Nguyen, Ngoc Tran Thi;Nor, Siti Azizah Mohd;Le, Thanh Hoa			
03F0E042C54EFF93FF67F9396317FBB7.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/12828492/files/figure.png	https://doi.org/10.5281/zenodo.12828492	Fig. 3. Detailed PhyML-phylogeny based on the analysis of the partial cox1 sequences (551 bp) showing the detailed relationships of the family Pangasiidae and related families (Austroglanididae, Ictaluridae, and Cranoglanididae). In total, 83 sequences, including 81 from Pangasius and Pangasianodon and 2 outgroup sequences from the order Gymnotiformes, were included (Table S3). The alignment was performed by MAFFT (Katoh and Standley 2013), curated by BMGE v1.12 (Criscuolo and Gribaldo 2010), the tree was reconstructed in PhyML 3.3 (Guindon et al. 2010) using a maximum likelihood method and 1000 bootstrap resamplings, and the output Newick tree was extracted and visualized using FigTree v1.4.4 (Rambaut 2018). The basal nodes of the Pangasiidae and two sister groups (Pangasianodon and (Pangasius + Helicophagus + Pseudolais)) are shown by arrows. The Pangasius mekongensis, Pangasianodon hypophthalmus, and Pangasius krempfi sequences in this study are bolded. The taxonmisidentified sequences were added with a question mark at the end. The taxa from Pangasiidae were shortened and those from other related families were presented with their full names. The abbreviations of the isolates are given in brackets, including the geographical origin or voucher records of each sequenced specimen (where available), which were retrieved from the previous studies (Karinthanyakit and Jondeung 2012; Tran and Duong 2019; Schedel et al. 2022). The country of origin or where the sample was reported is given in full or in brackets, if available. Accession numbers are given at the end of each sequence label. The scale bar represents the number of substitutions per site.	Fig. 3. Detailed PhyML-phylogeny based on the analysis of the partial cox1 sequences (551 bp) showing the detailed relationships of the family Pangasiidae and related families (Austroglanididae, Ictaluridae, and Cranoglanididae). In total, 83 sequences, including 81 from Pangasius and Pangasianodon and 2 outgroup sequences from the order Gymnotiformes, were included (Table S3). The alignment was performed by MAFFT (Katoh and Standley 2013), curated by BMGE v1.12 (Criscuolo and Gribaldo 2010), the tree was reconstructed in PhyML 3.3 (Guindon et al. 2010) using a maximum likelihood method and 1000 bootstrap resamplings, and the output Newick tree was extracted and visualized using FigTree v1.4.4 (Rambaut 2018). The basal nodes of the Pangasiidae and two sister groups (Pangasianodon and (Pangasius + Helicophagus + Pseudolais)) are shown by arrows. The Pangasius mekongensis, Pangasianodon hypophthalmus, and Pangasius krempfi sequences in this study are bolded. The taxonmisidentified sequences were added with a question mark at the end. The taxa from Pangasiidae were shortened and those from other related families were presented with their full names. The abbreviations of the isolates are given in brackets, including the geographical origin or voucher records of each sequenced specimen (where available), which were retrieved from the previous studies (Karinthanyakit and Jondeung 2012; Tran and Duong 2019; Schedel et al. 2022). The country of origin or where the sample was reported is given in full or in brackets, if available. Accession numbers are given at the end of each sequence label. The scale bar represents the number of substitutions per site.	2023-09-22	Duong, Thuy Yen;Pham, Linh Thi Khanh;Le, Xuyen Thi Kim;Nguyen, Ngoc Tran Thi;Nor, Siti Azizah Mohd;Le, Thanh Hoa		Zenodo	biologists	Duong, Thuy Yen;Pham, Linh Thi Khanh;Le, Xuyen Thi Kim;Nguyen, Ngoc Tran Thi;Nor, Siti Azizah Mohd;Le, Thanh Hoa			
03F0E042C54EFF93FF67F9396317FBB7.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/12828494/files/figure.png	https://doi.org/10.5281/zenodo.12828494	Fig. 4. Detailed PhyML-phylogeny based on the analysis of the partial cytB sequences (634 bp) showing the detailed relationships of the family Pangasiidae and related families (Austroglanididae, Ictaluridae, and Cranoglanididae). In total, 80 sequences, including 78 from Pangasius and Pangasianodon and 2 outgroup sequences from the order Clupeiformes, were included (Table S3). The alignment was performed by MAFFT (Katoh and Standley 2013), curated by BMGE v1.12 (Criscuolo and Gribaldo 2010), the tree was reconstructed in PhyML 3.3 (Guindon et al. 2010) using a maximum likelihood method and 1000 bootstrap resamplings, and the output Newick tree was extracted and visualized using FigTree v1.4.4 (Rambaut 2018). The basal nodes of the Pangasiidae and two sister groups (Pangasianodon and (Pangasius + Helicophagus + Pseudolais)) are shown by arrows. The Pangasius mekongensis, Pangasianodon hypophthalmus, and Pangasius krempfi sequences in this study are bolded. The taxonmisidentified sequences were added with a question mark at the end. The taxa from Pangasiidae were shortened and those from other related families were presented with their full names. The abbreviations of the isolates are given in brackets, including the geographical origin or voucher records of each sequenced specimen (where available), which were retrieved from the previous studies. The country of origin or where the sample was reported is given in full or in brackets, if available. Accession numbers are given at the end of each sequence label. The scale bar represents the number of substitutions per site.	Fig. 4. Detailed PhyML-phylogeny based on the analysis of the partial cytB sequences (634 bp) showing the detailed relationships of the family Pangasiidae and related families (Austroglanididae, Ictaluridae, and Cranoglanididae). In total, 80 sequences, including 78 from Pangasius and Pangasianodon and 2 outgroup sequences from the order Clupeiformes, were included (Table S3). The alignment was performed by MAFFT (Katoh and Standley 2013), curated by BMGE v1.12 (Criscuolo and Gribaldo 2010), the tree was reconstructed in PhyML 3.3 (Guindon et al. 2010) using a maximum likelihood method and 1000 bootstrap resamplings, and the output Newick tree was extracted and visualized using FigTree v1.4.4 (Rambaut 2018). The basal nodes of the Pangasiidae and two sister groups (Pangasianodon and (Pangasius + Helicophagus + Pseudolais)) are shown by arrows. The Pangasius mekongensis, Pangasianodon hypophthalmus, and Pangasius krempfi sequences in this study are bolded. The taxonmisidentified sequences were added with a question mark at the end. The taxa from Pangasiidae were shortened and those from other related families were presented with their full names. The abbreviations of the isolates are given in brackets, including the geographical origin or voucher records of each sequenced specimen (where available), which were retrieved from the previous studies. The country of origin or where the sample was reported is given in full or in brackets, if available. Accession numbers are given at the end of each sequence label. The scale bar represents the number of substitutions per site.	2023-09-22	Duong, Thuy Yen;Pham, Linh Thi Khanh;Le, Xuyen Thi Kim;Nguyen, Ngoc Tran Thi;Nor, Siti Azizah Mohd;Le, Thanh Hoa		Zenodo	biologists	Duong, Thuy Yen;Pham, Linh Thi Khanh;Le, Xuyen Thi Kim;Nguyen, Ngoc Tran Thi;Nor, Siti Azizah Mohd;Le, Thanh Hoa			
