taxonID	type	description	language	source
03C9645F5B5E982EFF48C7AAFAB9FD73.taxon	etymology	Etymology: based on the host species, from which the fungus was frequently isolated. Holotype: MFU 15 – 3563. Pathogen on leaves, fruits and shoots (Jayawardena et al. 2015). Conidiomata pycnidial in culture on PDA, globose to oval, solitary or aggregated in clusters, semi-immersed, black, 120 – 550 μm diam; exuding globose, black, glistening, conidial masses. Conidiophores indistinct, often reduced to conidiogenous cells. Conidiogenous cells discrete, fusiform, hyaline, cylindrical to subcylindrical or ampulliform to lageniform, rugose-walled, simple, 2 – 11 × 1 – 5 μm, apex 1 – 2 μm diam. Conidia fusoid, ellipsoid, straight to slightly curved, 4 - septate, (20 –) 22 – 28 (– 29) × (5 –) 6 – 9.8 (– 10.7) μm, x ± SD = 24.7 ± 1.4 × 7.7 ± 0.9 μm (n = 40), L / W ratio = 3.2, basal cell conic to acute with a truncate base, hyaline, rugose, thin-walled, 3 – 7 μm long, three-median cells, doliform, (13 –) 14 – 18 (– 19) μm long, x ± SD = 16.2 ± 0.9 μm, wall rugose, versicoloured, septa darker than the rest of the cell, somewhat constricted at the septa; second cell from base pale brown to olivaceous, 4.3 – 7.3 μm long; third cell olivaceous to darker brown, 4.1 – 7.6 μm long; fourth cell brown, 3.8 – 6.8 μm long; apical cell 2.5 – 4.8 μm long, hyaline, cylindrical to subcylindrical, thin and smooth walled, with 2 – 4 tubular apical appendages (mostly 3), arising from the apical crest, flexuous, unbranched, (14 –) 19 – 38.6 (– 43) μm long, x ± SD = 24.2 ± 4.5 μm, basal appendage 1 – 2, tubular, unbranched, centric, 2.2 – 7.2 μm long. Culture characteristics: Colonies on PDA reaching 50 – 60 mm in diam. after 7 d at 25 ° C, with lobate edge, whitish to pale honey coloured, with dense aerial mycelium on the surface with black, concentric conidiomata, reverse pale yellow. Habitat: On Vitis vinifera. Known distribution: China and India. Material examined: — CHINA. GuangXi Province: WuMing City, Pathogenic on leaves of Vitis vinifera cv. Summer Black, 18 December 2014, X. H. Li (MFLU 15 – 3563, holotype), ex-type culture, MFLUCC 15 - 1265 = KUMCC 15 - 0525; ibid cultures MFLUCC 15 - 1266, 15 - 1267, 15 - 1268, 15 - 1269, 15 - 1270, 15 - 1271, 15 - 1272, 15 - 1273, 15 - 1274; ICMP 20418, 20417, 20412. Notes: Neopestalotiopsis vitis is morphologically similar to its sister taxon N. australis. Conidiogenous cells of N. vitis are cylindrical to subcylindrical or ampuliform to lageniform and smaller (2 – 11 × 1 – 5 μm), while the conidiogenous cells of N. australis are ampuliform to lageniform and are larger (5 – 12 × 2 – 7 μm). Neopestalotiopsis australis has a single unbranched, centric basal appendage, while N. vitis has 1 – 2 unbranched, centric basal appendages. The apical cell of N. vitis conidia, are smaller (2.5 – 4.8 μm long) than the apical cell of N. australis conidia (3 – 6 μm long). Neopestalotiopsis egyptiaca is phylogenetically closely related to N. australis (Crous et al. 2015). However, this species differs from N. vitis by having larger conidiogenous cells (15 – 25 × 3 – 5 μm). Also, conidia of N. vitis are wider than those of N. egyptiaca. No additional isolates of N. australis are available to compare the morphological character variability. Based on the precedent set by Maharachchikumbura et al. (2014) for the species recognition of this genus comparing morphology and available gene sequence data we determined the species are to be distinct.	en	Jayawardena, Ruvishika S., Liu, Mei, Maharachchikumbura, Sajeewa S. N., Zhang, Wei, Xing, Qikai, Hyde, Kevin D., Nilthong, Somrudee, Li, Xinghong, Yan, Jiye (2016): Neopestalotiopsis vitis sp. nov. causing grapevine leaf spot in China. Phytotaxa 258 (1): 63-74, DOI: 10.11646/phytotaxa.258.1.4, URL: http://dx.doi.org/10.11646/phytotaxa.258.1.4
