identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
03C31A4EFFE41A74FFD7066A8A46FE4F.text	03C31A4EFFE41A74FFD7066A8A46FE4F.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Galleria mellonella (Buyukguzel and Kayaoglu 2014)	<div><p>2.1. G. mellonella culture</p> <p>Stock culture great wax moth G. mellonella L. (Lepidoptera: Pyralidae) was maintained by feeding newly hatched larvae on an artificial diet under laboratory conditions in an incubator (Nüve, FN 400) at 28 ± 2 °C, 65% ± 5% relative humidity, and continuous darkness. The artificial diet was composed of 420 g of bran, 150 mL of filtered honey, 150 mL of glycerol, 20 g of ground old dark honeycomb, and 30 mL of distilled water (Bronskil, 1961).</p> <p>2.2. Feeding experiments</p> <p>Oxyclozanide was purchased from Xi’an Quanao Biotech Co., Ltd. (Xian, Shaanxi, China). Oxyclozanide (2,3,5-trichloro-N-(3,5-dichloro-2-hydroxyphenyl)-6- hydroxybenzamide, powder, 99%, 401.46 g /mol) was directly incorporated into the diet as g of 100 g of diet (%). The oxyclozanide concentrations used in the present study were based on our previous study investigating the effects of oxyclozanide on the survival, development, and total protein of G. mellonella (Çelik et al., 2019). Four different concentrations, namely 0.003%, 0.03%, 0.3%, and 1.5% were tested. An artificial diet without oxyclozanide was used as the control group. First instar larvae were reared on artificial diets containing oxyclozanide concentrations to last instar larvae (7th) for comet assays. The experiments were performed in four replicates with five larvae for each replicate.</p> </div>	https://treatment.plazi.org/id/03C31A4EFFE41A74FFD7066A8A46FE4F	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Çelik, Cihat;Stanley B, David W.;B, Ender Büyükgüzel	Çelik, Cihat, Stanley B, David W., B, Ender Büyükgüzel (2023): Genotoxic effects of oxyclozanide on hemocytes of Galleria mellonella (Lepidoptera: Pyralidae) larvae. Turkish Journal of Zoology 47 (3): 147-154, DOI: 10.55730/1300-0179.3126, URL: http://dx.doi.org/10.55730/1300-0179.3126
