Psephellus sibiricus subsp. leaves, (L.) Wagenitz (L.) Wagenitz

3.4. Extraction and isolation of compounds from P. sibiricus leaves

The dried leaves of P. sibiricus ( 453 g) were crushed and soaked in MeOH. The MeOH extract was evapd, and the residue was dissolved in H 2 O. The aq. soln was re-extracted with CH 2 Cl 2. The CH 2 Cl 2 extract was dried with Na 2 SO 4, filtrated and evapd gave a residue ( 10.9 g) and chromatographed with CC method on silica gel with a mixtures of CH 2 Cl 2 and ( CH 3) 2 CO (ratio 45:1, 35:1 and 10:1) as eluent. The result was the isolation of the following compounds: scoparone ( 9) ( 12.7 mg, amorphous solid), scopoletin ( 10) ( 45.4 mg, m.p. 192–195 °C), umbelliferone ( 11) ( 63.7 mg, m.p. 230 °C), cynaropicrin ( 12) ( 6.7 mg, amorphous solid) and desacylcynaropicrin 8α-( Z)-(4′-hydroxy-2′-methyl) butenoate (]cebellin F) ( 13) ( 3.9 mg, amorphous solid). NMR data in Tables 7 View Table 7 and 8 View Table 8 .