Fusarium fujikuroi

Fusarium fujikuroi View in CoL species complex (FFSC) phylogeny

The eight isolates belonging to the FFSC were further analysed using a multi-gene phylogeny based on cmdA, rpb1, rpb2, tef1, and tub. The final alignment included 4 795 characters ( cmdA 545, rpb1 1534, rpb2 1551, tef 677 and tub 488) including align- ment gaps, and encompassed 54 isolates, with two outgroup taxa ( F. oxysporum CBS 716.74 and CBS 744.97) ( Table 2 View Table 2 ).

The analysis was consistently able to distinguish the three biogeographical clades known as the African,American and Asian clades sensu O’Donnell et al. ( 1998 a). All of the Indonesian isolates clustered within the Asian clade of FFSC except for isolate InaCC F991, identified as F. verticilloides , and clustered within the African clade ( Fig. 2 View Fig ). According to the multi-gene analysis, two isolates (InaCC F962 and InaCC F992) were identified as F. proliferatum , while two new phylogenetic species were recognised among the Indonesian isolates. Isolates InaCC F872 and InaCC F993, from central and East Java, respectively, clustered in a distinct, highly supported clade (96 bs/0.99 pp) closely related to F. mangiferae . Isolates InaCC F950–152, formed a distinct group (100 bs/1.0 pp), closely related to, but genetically distinct from F. sacchari .