Woolastookia rotundifrons (K. Viets, 1922)

Woolastookia rotundifrons (K. Viets, 1922)

Material examined — Portugal, Guarda: Seia, Rio Alva, Praia Fluvial de Sabugueiro , 40.401°N, 7.64°W, 1021 m a.s.l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2♂, 1♀ GoogleMaps

( BGE _00227_A01, BGE _00108_H06, BGE _00108_H08); Manteigas , Poio do Leão, 40.399°N, 7.541°W, 734 m a.s.l., 22 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1♀ ( BGE _00108_D05); Manteigas, Zêzere, Ponte dos Frades, 40.403°N, 7.526°W, 672 m a.s.l., 22 Aug. 2023, leg. Ferreira, Benitez-Bosco, Padilha & Stur 1♂ ( BGE _00108_D07), dissected and slide mounted ( RMNH) GoogleMaps ; Manteigas , Mondego, Covão da Ponte, 40.443°N, 7.514°W, 999 m a.s.l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1♀ ( BGE _00108_G02) GoogleMaps .

Remarks — The specimens from Portugal morphologically match the description of Woolastookia rotundifrons following Pešić et al. (2010). The sequenced specimens from Portugal form a unique BIN: BOLD:AFV8119, with the nearest neighbouring BIN being BOLD:AEE0289 (p -distance 17.39%), which includes specimens from Montenegro morphologically as well assigned to W. rotundifrons , indicating the need for taxonomic revision of this species from a wider geographical area.

Distribution — Central, southern and eastern Europe ( Pešić et al. 2010; Gerecke et al. 2016). New for Portugal.

Acknowledgements

Biodiversity Genomics Europe (Grant no.101059492) is funded by Horizon Europe under the Biodiversity, Circular Economy and Environment call (REA.B.3); co-funded by the Swiss State Secretariat for Education, Research and Innovation (SERI) under contract number 22.00173; and by the UK Research and Innovation under the Department for Business, Energy and Industrial Strategy’s Horizon Europe Guarantee Scheme.

SF was funded by the FCT through the program ‘Stimulus of Scientific Employment, Individual Support—3 rd Edition’ (https://doi.org/10.54499/2020.03526.CEECIND/CP1601/CP1649/CT0007). The authors would like to acknowledge Estação Biológica de Mértola, Mértola Municipality, CISE, Seia Municipality and Faculty of Sciences – University of Porto for the logistic support during the fieldwork and Laura Benitez-Bosco, Torbjørn Ekrem, Thomas S. Ekrem, Piotr Gadawski, Roman Hodunko, José Conde, Tiago Correia, António Rodrigues, Adriana Padilha and Rui Andrade for the great company during fieldwork. We thank Tom Goldschmidt (Munich) and Andrzej Zawal (Szczecin) whose constructive comments greatly improved this work.

References

Cantallo, H., Gomes, N., Antunes, C., Martins, D. & Costa, D.A. (2022) Checklist of water mites from mainland Portugal and its archipelagos. Zootaxa, 5213, 336–370.

Di Sabatino, A, Gerecke, R, Gledhill, T. & Smit, H. (2010) Acari: Hydrachnidia II. In: Gerecke, R. (Ed.), Chelicerata: Acari II. Süsswasserfauna von Mitteleuropa, Vol. 7, 2–2, Elsevier Spektrum Akademischer Verlag, Heidelberg, pp. 1–234.

Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high 679 throughput. Nucleic acids research, 32, 1792–1797. https://doi.org/10.1093/nar/gkh340

Esen, Y., Pešić, V. & Erman, O. (2011) Water mites of the family Aturidae Thor, 1900 from Turkey (Acari: Hydrachnidia), with description of two new species. Zootaxa, 2746, 25–42.

https://doi.org/10.11646/zootaxa.2746.1.3

Esen, Y., Pešić, V. & Erman, O. (2013) Water mites of the genus Brachypoda (Acari: Hydrachnidia: Aturidae) in Turkey. Zootaxa, 3686 (3), 326–334. https://doi.org/10.11646/zootaxa.3686.3.2

Felsenstein, J. (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39, 783 – 791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x

Gerecke, R., Marrone, F., Sorgi, G., Dossena, M. & Stoch, F. (2014) The water mites (Acari: Hydrachnidia) of the standing waters of Corsica, Sardinia and Sicily: review and new data. Italian Journal of Zoology, 81 (3), 389–408.

https://doi.org/10.1080/11250003.2014.922129

Gerecke, R., Gledhill, T., Pešić, V. & Smit, H. (2016) Chelicerata: Acari III. In: Gerecke R, ed. Süsswasserfauna von Mitteleuropa, Bd. 7/2 -3. Springer-Verlag Berlin, Heidelberg, pp. 1–429.

https://doi.org/10.1007/978-3-8274-2689-5

Kimura, M. (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.

Pešić, V., Erman, O. & Esen, Y. (2006) New records of water mites (Acari: Hydrachnidia) from Turkey. Acta entomologica serbica, 11 (1/ 2), 95–99.

Pešić, V., Smit, H., Gerecke, R. & Di Sabatino, A. (2010) The water mites (Acari: Hydrachnidia) of the Balkan peninsula, a revised survey with new records and descriptions of five new taxa. Zootaxa, 2586, 1–100. https://doi.org/10.11646/zootaxa.2586.1.1

Pešić, V., Zawal, A., Ferreira, S., Benitez- Bosco, L., Cruz-Oliveira, A., Girão, D., Padilha, A., Turaccio, P., Rossini, S., Ballini, L., Staffoni, G., Fratini, S., Ciofi, C., Iannucci, A., Ekrem, T., Stur, E. (2024) DNA barcode library of Portuguese water mites, with the descriptions of two new species (Acari, Hydrachnidia). Z ooKeys, 1217, 119–171.

https://doi.org/10.3897/zookeys.1217.131730

Smit, H. (2020) Water mites of the world with keys to the families, subfamilies, genera and subgenera (Acari: Hydrachnidia). Monografieën van de Nederlandse Entomologische Vereniging, 12, 1– 774.

Tamura, K., Stecher, G. & Kumar, S. (2021) MEGA 11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution, 38, 3022–3027.

https://doi.org/10.1093/molbev/msab120

Tuzovskij, P.V. (1978) K diagnozu Brachypoda (Hemibrachypoda) mutila (Walter, 1928) (Axonopsidae, Acariformes). Naucnye Doklady Vysshei Shkoly; Biologicheskie Nauki, 4, 47– 52. (in Russian)

Supplementary material 1

List of Aturidae View in CoL specimens

Data type: doc

Explanation note: List of water mite specimens of family Aturidae View in CoL , used for building the NeighbourJoining (NJ) tree ( Fig. 5 View Figure 5 ). Details on the DNA barcoded specimens from Portugal are given in Table 1. GenBank numbers are indicated by *. BINs are based on the barcode analysis from 15 December 2024.

Link: https://www.biotaxa.org/em/article/view/86633/81339