Dna

DNA reference barcodes

Mitochondrial CO1 sequences were obtained from 273 hyperiid specimens, representing 63 identified species in 34 genera and 16 families ( table 1, table S3). Family names are reported here as designated by Zeidler (2016). Amplification using universal CO1 primers failed for Hyperietta stebbingi Bowman, 1973 ; Iulopis loveni Bovallius, 1887 and Thamneus rostratus Bovallius, 1887 , and no sequence data were obtained for these species.

Results of genetic analyses and re-examination of morphological characters clarified original identifications for ten species reported in Burridge et al., (2017) for which species names were revised ( table 1). Specimens initially identified as Lanceola sp. 1 and Lestrigonus sp. 1 matched GenBank sequences for Lanceola sayana Bovallius, 1885 EF 989696.1 (100%) and Lestrigonus schizogeneios Stebbing, 1888 EF 989684.1 (98%), respectively, and were revised in this study. Specimens provisionally identified as Lycaea sp. 1 and Lycaea sp. 2 in Burridge et al., (2017) were genetically identical and failed to match any existing species description. Hence, they are here referred to as Lycaea sp. 1 . CO1 sequences from two Scina sp. individuals that were smaller than 0.5 mm matched with specimens identified as Scina tullbergi (Bovallius, 1885) (100%) and were re-named accordingly. Upon re-examination of morphological characters of Lycaeidae sp. 1 specimens, we identified these as members of the genus Simorhynchotus based on the unique formation of the last few segments of pereopod #2. Specimens identified as Vibilia stebbingi Behning & Woltereck, 1912 in Burridge et al., (2017) matched with South Atlantic Vibilia armata (99%) and were re-named. CO1 sequences of Brachyscelus sp. 1 , Lycaea sp. 1 and Simorhynchotus sp. 1 were highly distinct from those available for described species and are therefore provisionally indicated as distinct and potentially undescribed species. Finally, specimens of the genus Primno were primarily juveniles or subadults and therefore very difficult to identify based on underdeveloped morphological character- istics. However, adult specimens could be identified with confidence as Primno abyssalis (Bowman in Fulton, 1968), Primno brevidens Bowman, 1978 , Primno johnsoni Bowman, 1978 and Primno latreillei

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Calamorhynchus pellucidus Oxycephalidae Calamorhynchus 1 AMT22-47A SATL 1 AFL1397 NBCHyAmp-012

pellucidus

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Hyperoche medusarum Hyperiidae Hyperoche 1 AMT22-42 WTRA 1 AFK6823 NBCHyAmp-033

medusarum

CONTRIBUTIONS TO ZOOLOGY 94 (2025) 261–291

CONTRIBUTIONS TO ZOOLOGY 94 (2025) 261–291

Phronimopsis spinifera Lestrigonidae Phronimopsis 17 AMT22-29A, NATR, 2 AEK4936, NBCHyAmp-077-

spinifera AMT 22-35, WTRA, AFJ1834, 093

AMT22-45, SATL AFJ1835,

AMT22-47A, AFJ1837,

AMT22-58A, AFJ1833,

AMT22-60A AFJ1834,

AFJ1838,

AFJ1836

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Primno brevidens Phrosinidae Primno johnsoni 2 AMT22-31, NATR, 2 AFK9803, NBCHyAmp-155-

AMT22-62 SATL- n.a. 156

SSTC

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Simorhynchotus sp. 1 Lycaeidae Lycaeidae sp. 1 2 AMT22-35, WTRA 2 ADO9548, NBCHyAmp-222 -

AMT22- 45 n. a. 223

CONTRIBUTIONS TO ZOOLOGY 94 (2025) 261–291

CONTRIBUTIONS TO ZOOLOGY 94 (2025) 261–291

CONTRIBUTIONS TO ZOOLOGY 94 (2025) 261–291

Stebbing, 1888 and by combining morphological and genetic information, we revised some of the species names with respect to Burridge et al., (2017) (see table 1).