Sutera cordata

3.5. Sutera cordata View in CoL

Fresh plants ( 43 g) were blended with EtOH (200 ml) and treated as above to give after concentration 1.57 g residue. Chromatography gave: a fraction containing mainly secologanoside ( 14, 520 mg), a fraction with mainly secologanic acid ( 13, 70 mg), a mixture (1:1) of sweroside ( 12) and villoside ( 11) ( 33 mg), echinacoside ( 16, 22 mg), followed by fractions (total 300 mg) which were repeatedly chromatographed over C 18 HPLC (MeCN-H 2 O) to yield: tubuloside A ( 18, MeCN–H 2 O 15:85, 2 mg) and 2′′′′-acetyl angoroside A ( 19, MeCN–H 2 O 21:79, 6 mg), both in a semi-pure state. Villoside ( 11, 5.9 mg) and sweroside ( 12, 6.0 mg) were separated by HPLC with MeCN-H 2 O (20:80). Pure samples of ( 13, 20.8 mg) and ( 14, 5.2 mg) were also obtained with MeCN-H 2 O gradient (17:83 → 85:15, v/v, over 15 min).