Diadiplosis coccidarum ( Cockerell, 1892 ), 1911

Diadiplosis coccidarum ( Cockerell, 1892)

Diplosis coccidarum Cockerell, 1892: 181. Jamaica.

Lobodiplosis coccidarum Felt 1911: 195. St. Vincent.

Karschomyia cocci Felt 1913: 304. Puerto Rico: Patillas.

Larva third instar. Body elongated and cylindrical, length 2.3 mm (n=5). Integument rough. Head capsule triangular ( Fig. 2a View Figure 2 ), as long as posterolateral apodemes. Antennae long, about half the length of the head. Prothoracic spatula ( Fig. 2b View Figure 2 ): 0.12 mm long (n=5), bidentate with triangular teeth, and stalk with posterior region slightly enlarged. Two groups of three lateral papillae on each side of the spatula (one papilla asetose and a pair of papillae setose in each group). Two sternal papillae on all thoracic segments and four sternal papillae on first to seventh abdominal segment, each without seta and raised on mamelons. Two pleural papillae with seta, present on each side of every thoracic and abdominal segment, particularly close to the spiracle on the abdominal segments. Six dorsal papillae on all thoracic and first to seventh abdominal segments, each with seta. Two ventral papillae on all thoracic segments adjacent to lateral papillae and four ventral papillae on the first to seventh abdominal segments on discrete mamelons, each without seta. Eighth abdominal segment with two setose dorsal papillae and two ventral papillae without seta. Terminal segment ( Figs. 2 View Figure 2 c-d): Two pairs of terminal papillae with seta, innermost pair slightly longer than outermost pair; ventral cleft anus with two anal papillae without seta, and a ventroapical lobe on each side.

Pupa. Body length 2.3 mm long (n=8). Head ( Fig. 3a View Figure 3 ): antennal bases ventrally rounded, with a triangular extension anteromedially, a small rounded projection like a denticle medioventrally and one asetose antennal papilla; cephalic setae 0.16 mm long (n=13) with an adjacent papilla asetose on a conspicuously raised base; two lower facial papillae on each side (one setose and the other one asetose), one group on each side of three lateral facial papillae (one with seta and two asetose), and one pair of asetose upper lateral facial papillae located on the upper cephalic margin slightly sclerotized. Thorax: prothoracic spiracle elongated and well developed, 0.10 mm long (n=14); wing reaching the basal third of the third abdominal segment; first pair of legs reaching midlength of the fourth abdominal segment, second pair of legs reaching posterior margin of the fourth abdominal segment and third pair reaching posterior margin of the fifth abdominal segment. Abdomen ( Fig. 3b View Figure 3 ): segments two through eight with several rows of simple, tiny, and conspicuous spines at the proximal one-third of the dorsal region; segments two to six with elongated lateral spiracle, 0.04 mm long (n=24); first to seventh terga with four posteromedial asetose papillae, one pair of outermost setose papillae, and a pair of anterior trichoid sensilla; first to seventh pleura with two closely spaced lateral papillae with short seta on each side, one of them directly below of spiracle when present; eighth tergum with a pair of proximal trichoid sensilla, and a pair of distal setose papillae.

Additional notes on adults: Head: Antenna: total length 1.10 mm in male (n=3) and 0.81 mm in female (n=2); scape broader distally, length 0.04 mm (n=14) and pedicel globose, length 0.03 mm (n=14) in both sexes; male flagellomeres binodal and tricircumfilar, basal and distal circumfila of each flagellomere with regular loops in length, medial circumfila slightly shorter than distal and basal loops; female flagellomeres cylindrical, circumfila with two ring connected horizontal and interconnected mesially forming an x shape; flagellomeres necks bare, first and second flagellomeres connate in both sexes and twelfth flagellomere with apical process ( Fig. 4a View Figure 4 ). Frontoclypeus with 13 setae in male and female (n=6). Labrum triangular, with 2 pairs of setae. Hypopharynx of same shape as labrum, with anteriorly directed lateral setulae. Labella hemispherical-convex. Palpus total length 0.14 mm (n=4), palpus 4-segmented, all setose, first globoid, and second to fourth cylindrical. Thorax: scutum with two dorsocentral rows of setae, two rows of lateral setae; scutellum with scattered setae. Anepimeron with 7 setae in male (n=8) and female (n=2), other pleural sclerites bare. Legs: protarsal claw 1-toothed and bend near midlength, meso and meta tarsal claw simple, empodium reaching claw bend ( Figs. 4 View Figure 4 b-c). Wings: 1.20 mm long (measured from the arculus to the apex) and 0.53 mm wide in male (n=10), and 1.35 mm long and 0.60 mm wide in female (n=4), R 5 vein almost straight and joining C at wing apex. Abdomen: Second to seventh tergites rectangular, completely sclerotized, with a complete row of posterior setae and several scattered setae in males and females, eighth tergite not sclerotized in both sexes, basal pair of trichoid sensilla present at tergites third to eighth in males and third to seventh in females. Sternites two to seven rectangular, sclerotized except for two ellipsoidal and lateral patches, with two rows of setae one posterior and the other one mesally, eighth sternite not sclerotized and sternites three to seven with basal and central pair of trichoid sensilla in both sexes. Male terminalia ( Fig. 4e View Figure 4 ): Gonocoxites elongated, parallel, and setulose with a posterior extension apically tapered, extending beyond the gonostylus insertion, distal margin covered with robust setae and some scattered setae dorsally, 0.11 mm long and 0.04 mm wide (n=6); gonostylus rectangular, with a length two times its width, covered with microtrichia and a few distal setae, pectinate apical tooth, 0.05 mm long and 0.02 mm wide (n=5); cerci bilobed, almost the same length as the hypoproct, covered with microtrichia and scattered setae on the distal half, lobes triangular and apically rounded; hypoproct bilobed, narrower proximally, completely covered with microtrichia and some scattered setae dorsally ( Fig. 4d View Figure 4 ), lobes apically rounded, with a U-shaped reentrance; aedeagus elongated and rounded apically, longer than hypoproct and cerci. Ovipositor: 0.09 mm long (from the basal margin of segment nine to the cerci apex) (n=1), tergite nine with a pair of setae longer than the others, located in the distal third; cerci elongated, with several scattered setae, 0.05 mm long (n=1); hypoproct elongated, and covered with microtrichia.

Material Examined. 5 larvae of third instar ( LEUA: 66504-05 / MZUSP) and 1 pupal exuviae ( MZUSP), Colombia, Huila, Rivera, Vereda Llanitos, Finca La Florida ; 02°46’48”N, 75°14’07”W; 819 meters of altitude; 24.ix.2021; E. Ospina leg.; collected and reared from infestation of Saccharicoccus sacchari ( Hemiptera : Pseudococcidae ) on Saccharum officinarum ( Poaceae ); 5 pupal exuviae ( LEUA: 66506- 08 / MZUSP) GoogleMaps , 1 ♀ ( MZUSP) and 5 ♂ ( LEUA: 66509-11 / MZUSP) with the same previous data, except for the emergence: 08.x.2021 GoogleMaps ; 1 ♂ ( MZUSP) with same data and emergence: 04.x.2021; and GoogleMaps 1 ♀ ( LEUA: 66512 ) and 2 pupal exuviae ( LEUA: 66513 / MZUSP) with same data, except for the emergence: 22.x.2021. GoogleMaps All material, with one specimen per slide-mounted, is deposited in the Laboratory of Entomology of the University of the Amazonia (LEUA), Florencia, Caquetá, Colombia in the Central Taxonomic Collection (CTC), and the Diptera Collection of Museum of Zoology of the University of São Paulo (MZUSP), Brazil.