Wolbachia

Wolbachia View in CoL infection in Cx. tritaeniorhynchus

Wolbachia detection was initially performed by conducting PCR for the wsp gene. If Wolbachia was not detected, a second PCR targeting the 16S rDNA region was conducted. If amplification also failed in the second PCR, the result was considered to be negative, i.e. no

Wolbachia DNA. Te results from 12 regions of the ROK indicated that Wolbachia was detected in 32 (10.2%) of the 313 individuals of Cx. tritaeniorhynchus analyzed ( Table 3). All 32 Wolbachia -positive individuals were from Ct-J, with none detected in the eight individuals from Ct-C.

Wolbachia was identified in populations from five of the 12 regions. Phylogenetic analysis using ML based on the wsp sequence of Wolbachia from the infected mosquitoes confirmed the presence of supergroup A and supergroup B ( Fig. 5 View Fig ). Among the 32 mosquitoes that tested positive for Wolbachia , two were infected with supergroup A and 30 were infected with Supergroup B. Supergroup A was found in Gimhae and Chungju, while supergroup B was identified in Gimhae, Chungju, Daegu (both in 2022 and 2023), Haenam and Wanju. Both supergroup A and supergroup B were present in Gimhae and Chungju. However, none of the 32 mosquitoes were found to be co-infected with both supergroup A and subgroup B.