Vishniacozyma diospyri C. Y. Chai & F. L. Hui, 2025

Vishniacozyma diospyri C. Y. Chai & F. L. Hui sp. nov.

Figs 2 A, F View Figure 2

Etymology.

The specific epithet diospyri refers to Diospyros, the name of the genus from which the type strain was isolated.

Typus.

China. • Henan Prov.: Neixiang Co., Baotianman Nature Reserve (33°29'07"N, 111°52'51"E), Sep 2022, J. Z. Li, in the phylloplane of Diospyros lotus , NYNU 221044 (holotype CICC 33574 T , GenBank: OP 954624, OP 954569 ); culture ex-type PYCC 9955 View Materials GoogleMaps .

Description.

On YM agar after seven days at 20 ° C, the streak culture is yellowish-cream, butyrous, and smooth, with an entire margin. After three days in YM broth at 20 ° C, cells are ovoid, ellipsoidal, and cylindrical, 2.5–4.4 × 4.1–13.7 μm, and single; budding is polar. After one month at 20 ° C, a ring and sediment are present. In Dalmau plate culture on CMA, pseudohyphae and hyphae are not formed. Sexual structures are not observed on PDA, CMA or V 8 agar. On corn meal agar, ballistoconidia are not produced. Glucose fermentation is absent. Glucose, sucrose, raffinose, melibiose, galactose, lactose, trehalose, maltose, melezitose, methyl-α-D-glucoside, cellobiose, salicin (weak), L-sorbose, L-rhamnose, D-xylose, L-arabinose, D-arabinose, 5 - keto-D-gluconate, D-ribose, glycerol (delayed), erythritol, ribitol (delayed), galactitol, D-mannitol, D-glucitol, myo-inositol, DL-lactate, succinate, D-gluconate, D-glucosamine, N-acetyl-D-glucosamine, 2 - keto-D-gluconate, D-glucuronate, and glucono- 1, 5 - lactone are assimilated as sole carbon sources. Inulin, methanol, and ethanol are not assimilated. Nitrite and L-lysine are assimilated as sole nitrogen sources. Nitrate, ethylamine, and cadaverine are not assimilated. Maximum growth temperature is 25 ° C. Growth in vitamin-free medium is positive. Growth on 50 % (w / w) glucose-yeast extract agar is negative. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive.

Additional strain examined.

China. • Henan Prov.: Neixiang Co., Baotianman Nature Reserve (33°29'07"N, 111°52'51"E), in the phylloplane of Cornus officinalis , Sep 2022, J. Z. Li, NYNU 2211329 (GenBank: PQ 496711 , PQ 496709 ) GoogleMaps .

Note.

In the phylogenetic analyses, V. diospyri formed a separate branch that clustered with V. catalpae with high support (BS / 99, BPP / 1.0; Fig. 1 View Figure 1 ). However, V. diospyri differs from V. catalpae by 20 nucleotides (12 / 486 in ITS and 8 / 559 in LSU) and by its ability to assimilate D-arabinose, succinate, and D-glucosamine and its inability to assimilate inulin, ethylamine, and cadaverine.