Dna extraction
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publication ID |
https://doi.org/10.11646/phytotaxa.716.3.6 |
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persistent identifier |
https://treatment.plazi.org/id/4A1FCB5D-FFE8-FF8A-4180-FCF4FC9DFBD6 |
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treatment provided by |
Felipe |
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scientific name |
Dna extraction |
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DNA extraction , amplification and sequencing
Genomic DNA was extracted using 2×CTAB solution (Coolaber Technology Co., Ltd., Beijing, China) following the manufacturer’s instructions. The internal transcribed spacer (ITS), the large subunit of nuclear ribosomal RNA (nrLSU), the second-largest subunit of RNA polymerase II ( rpb2) and translation elongation factor 1-alpha ( tef1- α) were amplified with primers ITS1/ITS4, LROR/LR5, bRPB2-6F/RPB2-7R and 983F/1567R ( White et al. 1990, Vilgalys lab 1992, Liu et al. 1999, Matheny 2005, Rehner & Buckley 2005), respectively. The PCR products were sequenced by Sangon Bioengineering ( Shanghai) Co., Ltd.
No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.