Pseudamnicola bodoni Delicado, Boulaassafer & Hauffe, 2025

Pseudamnicola bodoni Delicado, Boulaassafer & Hauffe sp. nov.

( Figs 9 View Figure 9 , 10 View Figure 10 )

Pseudamnicola sp. 5 — Delicado et al. 2015

Pseudamnicola sp. 3 — Delicado et al. 2018

Pseudamnicola sp. 3 — Boulaassafer et al. 2020

ZooBank registration: urn:lsid:zoobank.org:act:536D395B-2699-420F-B2A6-8020BC0BC931

Etymology

Named after Marco Bodon, collector of the species, in recognition of his valuable contribution to the knowledge of the molluscan fauna of Italy.

Type material

Holotype ( MNCN 15.05 About MNCN /200502H) in the MNCN collection and five paratypes in the UGSB collection ( UGSB 988 ). Type locality: a spring on the left bank of the Biedano Stream , Blera, Latium, Italy.

Material studied

A spring on the left bank of the Biedano Stream , Blera, Latium, Italy, 42.2680°N, 12.0256°E, leg. M.B., November 2007, MNCN 15.05 About MNCN /200502H and UGSB 988 (80% ethanol). GoogleMaps A spring in Via Marrucella, Capri Island, Italy, 40.5492°N, 14.2323°E, leg. M.B., March 2004, UGSB 5967 (80% ethanol). GoogleMaps A stream near Sperlonga, Latium, Italy, 41.2579°N, 13.4348°E, leg. M.B. and S.C., January 2014, UGSB 16628 (80% ethanol) GoogleMaps .

Diagnosis

Shell small, ovate-conic, with a short spire; protoconch microsculpture pitted; central radular tooth formula 4-C-4/1-1; bursa copulatrix small, pyriform, with a duct slightly longer than bursal length; SR1 elongate, with a short duct; penis gradually tapering, unpigmented, with many folds over the entire surface and a wide base; nervous system darkly pigmented, elongate (mean RPG ratio =.50).

Description

Shell ovate-conic, whorls 3–3.5, height 2–2.5 mm ( Fig. 9A–C View Figure 9 ; Supporting Information, Table S3). Periostracum yellowish. Protoconch ~400 µm wide, whorls 1.5; nucleus ~125 µm wide; protoconch microsculpture pitted ( Fig. 9G View Figure 9 ). Teleoconch whorls moderately convex, with deep sutures; body whorl large, occupying about three-quarters of total shell length. Aperture longer than wide, slightly oval; inner lip thicker than outer lip; peristome margin simple, straight ( Fig. 9D View Figure 9 ). Umbilicus narrow, not covered by the inner lip. Holotype: SL = 2.21 mm, SW = 1.81 mm, AL = 1.26 mm, and AW = 0.86 mm.

Operculum oval, brownish, about two and a half whorls; muscle attachment area oval and located near the nucleus ( Fig. 9E, F View Figure 9 ).

Radular length intermediate, ~600 µm (~25% of total shell length), with ~60 rows of teeth ( Fig. 9H View Figure 9 ). Central tooth formula 4-C-4/1-1 ( Fig. 9I View Figure 9 ); basal tongue V-shaped, length about equal to lateral margin. Lateral tooth formula 3-C-3. Inner marginal teeth having ~25 tapered cusps, shortening towards the base. Outer marginal teeth with ~20 sharp cusps ( Fig. 9J View Figure 9 ).

Animal darkly pigmented except for neck and tentacles ( Fig. 10F View Figure 10 ). Snout as long as wide, with medium distal lobation; foot size intermediate, with dorsal pigmentation. Ctenidium with 17–19 well-developed gill filaments, occupying ~50% of pallial cavity length and posteriorly positioned. Osphradium of intermediate width and opposite middle of ctenidium ( Fig. 10A View Figure 10 ). Stomach as long as wide, with two chambers almost equal in size and a medium-sized posterior caecum; style sac longer than wide, surrounded by an unpigmented intestine ( Fig. 10B View Figure 10 ; Supporting Information, Table S4). Nervous system elongate (mean RPG ratio =.50), darkly pigmented, darker on ganglia than on connectives and commissures; cerebral ganglia approximately equal in size ( Fig. 10C View Figure 10 ).

Female glandular oviduct approximately four times as long as wide. Albumen gland shorter than capsule gland. Bursa copulatrix small, pyriform, longer than wide. Bursal duct longer than bursal length. Renal oviduct black-pigmented until the area above the insertion of seminal receptacle, making one or two loops. Seminal receptacle elongate, with a short duct, joining renal oviduct at the insertion point with bursal duct ( Fig. 10D, E View Figure 10 ; Supporting Information, Table S5).

Male genitalia with a prostate gland approximately three times as long as wide, bean-shaped; seminal duct entering the middle-posterior region; pallial vas deferens emerging close to its anterior edge ( Fig. 10H View Figure 10 ). Penis shorter than head, gradually tapering, unpigmented, with many folds over the entire surface ( Fig. 10F, G View Figure 10 ; Supporting Information, Table S6); base wide; attached well behind the right eye; penial duct narrow, curved, coursing close to outer edge.

Habitat

The species lives in coastal running waters, mainly in small springs.

Remarks

The few individuals available for the study showed little variation in shell features ( Fig. 9A–C View Figure 9 ). The species was first discovered by DNA-based phylogenetic analyses ( Delicado et al. 2015), but the comprising populations could not be assigned to any of the nominal species owing to a lack of morphological information. In our study, all DNA-based species delimitation methods (except the ABGD for 16S) recovered this species as distinct from P. lucensis , P. orsinii , and P. argentariensis , which also occur on the Apennine Peninsula and adjacent islands ( Issel 1866, Giusti 1976, Falkner and Boeters 2003). Pseudamnicola bodoni differs from these geographically proximate species in the combination of a short spire, short seminal receptacle, short penis, and average sequence divergence of 4.8%–7.8% for COI. It differs from the Sicilian populations of P. moussonii and P. reticulatus in having a more globose shell, a larger number of cusps on the marginal radular teeth, a shorter penis, and an average sequence divergence of 6.1%–7.6% for COI.