Vishniacozyma tianchiensis C. Y. Chai & F. L. Hui, 2025

Vishniacozyma tianchiensis C. Y. Chai & F. L. Hui sp. nov.

Fig. 2 E View Figure 2

Etymology.

The specific epithet tianchiensis refers to the geographic origin of the type strain, Tianchi Mountain, Songxian County, Henan Province.

Typus.

China. • Henan Prov.: Songxian Co., Tianchi mountain (34°32'27"N, 112°16'39"E), Jun 2023, in the phylloplane of Salix matsudana , J. Z. Li, NYNU 236163 (holotype CICC 33617 View Materials T, GenBank: OR 426458 , OR 426457 ); culture ex-type PYCC 9988 View Materials GoogleMaps .

Description.

On YM agar after seven days at 20 ° C, the streak culture is yellowish cream, mucoid, glistening, and smooth, with an entire margin. After three days in YM broth at 20 ° C, cells are globose, 3.7–5.4 × 4–6.4 μm, and single; budding is polar. After one month at 20 ° C, a ring and sediment are present. In Dalmau plate culture on CMA, pseudohyphae and hyphae are not formed. Sexual structures are not observed on PDA, CMA or V 8 agar. On corn meal agar, ballistoconidia are not produced. Glucose fermentation is absent. Glucose, inulin, sucrose, raffinose, melibiose, galactose, lactose, trehalose, maltose, methyl-α-D-glucoside (delayed), cellobiose, salicin (weak), L-sorbose, L-rhamnose, D-xylose, L-arabinose, D-arabinose (weak), 5 - keto-D-gluconate, D-ribose, glycerol (weak), erythritol, ribitol, galactitol, D-mannitol, D-glucitol, myo-inositol, DL-lactate, succinate, D-gluconate, D-glucosamine (delayed and weak), N-acetyl-D-glucosamine (weak), 2 - keto-D-gluconate, D-glucuronate, and glucono- 1, 5 - lactone are assimilated as sole carbon sources. Melezitose, methanol, ethanol, and citrate are not assimilated. Nitrate, nitrite, ethylamine, L-lysine, and cadaverine (weak) are assimilated as sole nitrogen sources. Maximum growth temperature is 25 ° C. Growth in vitamin-free medium is positive. Growth on 50 % (w / w) glucose-yeast extract agar is positive. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive.

Additional strain examined.

China. Henan Prov.: Xixia Co., Funiu Mountain (33°20'45"N, 111°47'37"E), Oct 2023, in the phylloplane of Diospyros lotus , S. Liu & Y. Z. Qiao, NYNU 2311236 (GenBank: PQ 496716 , PQ 496715 ) GoogleMaps .

Note.

In the phylogenetic analyses, V. tianchiensis was grouped with V. dimennae (Fell & Phaff) X. Z. Liu, F. Y. Bai, M. Groenew. & Boekhout , V. globispora (B. N. Johri & Bandoni) X. Z. Liu, F. Y. Bai, M. Groenew. & Boekhout , and V. pseudodimennae in a clade with high support values (BS / 100, BPP / 1.0; Fig. 1 View Figure 1 ). V. tianchiensis differs from V. dimennae by 21 nucleotides (14 / 479 in ITS and 7 / 596 in LSU), from V. globispora by 40 nucleotides (28 / 478 in ITS and 12 / 596 in LSU), and from V. pseudodimennae by 37 nucleotides (22 / 437 in ITS and 15 / 566 in LSU). Physiologically, V. tianchiensis can be differentiated from three closest known species, by the ability to assimilate inulin, melibiose, methyl-α-D-glucoside, and erythritol and the inability to grow at 30 ° C.