Gossypium

2.3. GHQ (9) and gossypol (1) in the D genome of Gossypium View in CoL View at ENA and in commercial cottons

G. thurberi is a member of the D genome of Gossypium . In a previous investigation of terpenoids in the young leaves of G. thurberi using TLC plates, only gossypol (1) was identified in this tissue ( Stipanovic et al., 1977). Thus, terpenoids such as the sesquiterpenoid hemigossypolone (7) and the sesterterpenoids heliocides H 1, H 2, H 3 and H 4 (3–6) ( Fig. 1 View Fig ) that are found in commercial Upland cottons ( Gossypium hirsutum ), as well as other members of Gossypium , were not found. To extend this investigation, several Gossypium accessions of the D genome available in the USDA Cotton Germplasm collection were grown in the greenhouse; leaves were collected, freeze dried, and ground. The ground tissue was extracted and immediately subjected to HPLC analysis; the results are shown in Table 3 View Table 3 . As found previously, other sesquiterpenoids and sesterterpenoids normally in G. hirsutum were not detected in G. thurberi nor in most other members of the D genome. However, GHQ (9) was present in small amounts in these cottons. As previously reported ( Stipanovic et al., 1977), within the D genome only the leaves of Gossypium gossypioides (Ulbrich) contained hemigossypolone (7) and heliocides (3–6); the leaves of other members of the D genome did not contain these terpenoids. In addition, the leaves of G. raimondii contained only gossypol (1) and the sesquiterpenoid, raimondal (8) ( Fig. 1 View Fig ). Four commercial cultivars of G. hirsutum cotton also were analyzed ( Table 4 View Table 4 ). GHQ (9) was not detected in these plants, but, as expected, gossypol (1), hemigossypolone (7) and heliocides H 1 – H 4 (3–6) were detected.