Didymocyrtis shanxiensis H. Y. Wang & Y. F. Han, 2025

Didymocyrtis shanxiensis H. Y. Wang & Y. F. Han sp. nov.

Fig. 4 View Figure 4

Etymology.

shanxiensis, referring to Shanxi province where the type locality was isolated.

Type.

China • Shanxi Province, Datong City , sewage treatment plant (40°2'42"N, 113°20'48"E), soil, August 2021, Yulian Ren, ex-type culture GZUIFR 24.004 , dried holotype GZAC 24.004 About GZAC . ITS sequences, GenBank PQ 065635 ; tub 2 sequences, GenBank PQ 119783 GoogleMaps .

Description.

Culture characteristics (7 days of incubation at 25 ° C): Colony on PDA, 30–35 mm diam., thin, villiform, cream ( RAL 9001), reverse cream ( RAL 9001), regular in the margin; Colony on MEA, 20–25 mm diam., thick, villiform, light lvory ( RAL 1015), reverse dahlia yellow ( RAL 1033), regular in the margin; Colony on OA, 30–35 mm diam., texture velvety, olive yellow ( RAL 1020), reverse stone gray ( RAL 7030), regular in the margin. Black spots produced after incubating 15 days on PDA.

On PDA medium after 30 days of incubation at 25 ° C, Hyphae septate, hyaline, smooth, thick-walled, 1.0–2.5 μm wide. Conidiomata submersed, brown to black, globose, 150–250 µm diam. Conidiophores reduced to conidiogenous cells. Conidiogenous cells globose to subglobose, also ampulliform, aseptate, hyaline, smooth, thick-walled, 4.5–10.0 × 2.0–6.0 μm (mean ± SD = 7.0 ± 1.9 × 3.5 ± 1.0 μm, n = 15). Conidia abundant, cymbiform mostly, brown, smooth, apex subobtuse, base truncate, 1 - septate, 5.0–11.0 × 1.5–3.0 μm (mean ± SD = 7.5 ± 1.6 × 2.0 ± 0.4 μm, n = 30).

Additional specimens examined.

China • Shanxi Province, Datong City , sewage treatment plant (40°2'42"N, 113°20'48"E), soil, August 2021, living cultures GZUIFR 24.005 . ITS sequences, GenBank PQ 065636 ; tub 2 sequences, GenBank PQ 119784 GoogleMaps .

Notes.

Twenty-nine species of the genus Didymocyrtis are recorded in the Index Fungorum. However, the DNA sequence data of fifteen species have no records in NCBI database. Phylogenetically, our two strains ( GZUIFR 24.004 and GZUIFR 24.005 ) clustered in a single clade with a high support value (ML / BI 98 / 1) (Fig. 3 View Figure 3 ). In the phylogenetic tree, although our new species D. shanxiensis and Didymocyrtis brachylaenae Crous are closely related species, they were obviously different in morphological characteristics. Didymocyrtis shanxiensis , having conidiophores reduced to conidiogenous cells, globose to subglobose and ampulliform conidiogenous cells, and cymbiform conidia, can be distinguished from D. brachylaenae with subcylindrical and branched conidiophores, lining the inner cavity and ampulliform to doliiform conidiogenous cells, and fusoidellipsoid to subcylindrical conidia ( Crous et al. 2018).