Aeonium palmense

Aeonium palmense View in CoL

Aeonium palmense Aeonium palmense Rubus sp.

Stachys sylvatica Atropa bella-donna Salvia glutinosa Urtica dioica Lycopus aeropaeus Stachys alpina Solanum lycopersicum Solanum lycopersicum Solanum lycopersicum Hyoscyamus albus on Host plant monspeliensis Cistus Cistus monspeliensis Cistus symphytifolius Cistus monspeliensis Fabaceae Fabaceae . Silene sp Silene . sp Solanum lycopersicum Silene sp. Silene sp. Pteridium aquilinum aquilinum Pteridium W W W W E E W E E ’’ ’’ ’’ ’’ ’’ ’’ E E ’’ ’’ ’’ E E 08 ’ ’05 05 ’ 06 ’ 58 ’ 58 ’ ’’’ 18 ’’ 54 ’ 30 16 ’ 16 ’ 05’’ ’’ 05 Long 16 52 º º 55 17 55 º 17 º 17 17 23 º 13 23 º 13 º 7 27 ’ º 03 7 34 º 15 00 º 14 º 14 00 ’ 17 7 º ’ 17 7 º N N N N N N N N N N N N N ’’ ’’ ’’ ’’ ’’ ’’ ’’ ’’ ’’ ’’ ’’ ’’ ’’ 12 32 32 36 19 19 03 ’ 18 31 49 49 53 53 ’ ’ ’ ’ ’ ’ ’ ’ ’ ’ ’ ’ 20 47 47 07 56 56 02 20 08 54 54 01 01 Lat 28 º 28 º 28 º º 28 37 º 37 º º 45 º 44 º 28 º 49 º 49 45 º 45 º.. Rep Rep Country Spain Spain Spain Spain Italy Italy Italy Italy Spain Czech Czech Italy Italy CI CI CI CI,, Province Tenerife CI, Palma La,, La Palma La Gomera Sicilia Sicilia Torino Cuneo. Gr Canaria Stredocesky Stredocesky Torino Torino Year 2009 2009 2009 2009 2007 2007 2009 2008 2008 2009 2009 2009 2009. 12S 274728 274729 274730 274731 274732 274733 274771 274772 274768 274769 274770 274798 274799 Acc KY KY KY KY KY KY KY KY KY KY KY KY KY. 16 S 274653 274654 274655 274656 274657 274658 274695 274696 274692 274693 274694 274722 274723 Acc KY KY KY KY KY KY KY KY KY KY KY KY KY COI. 274576 274577 274578 274579 274580 274581 274614 274615 274611 274612 274613 274647 274648 Acc KY KY KY KY KY KY KY KY KY KY KY KY KY 1 - 1 2 1 1 1 1 1 2 1 1 - code 13 -

C C

- 15

C

- 16 -

C

17

D

69 - 69

D

- - 82

D

-

D

83 102

D D

151 151 -

D

1

B

1 - 1

B

2 -

Continued * * *

1

.

Table

Species Cyrtopeltis canariensis Dicyphus albonasutus Dicyphus globulifer Monalocoris filicis *

the concatenated sequences partitioned by single genes, set at 20 million generations with sampling and diagnosis frequency every 1000 generations. The burn-in was set at 30% of sampled trees. Convergence was confirmed by standard deviation of split frequencies approaching zero (<0.0001) and stabilization of likelihood values. ML analysis was performed on the same partitioned dataset using RAxML with 1000 bootstraps ( Stamatakis, 2014). Trees were visualized using FIGTREE v.1.4.2 (http:// tree.bio.ed.ac.uk/software/figtree/).

Discrimination of species within Dicyphus (Dicyphus) was assessed in part on genetic distances ( Hebert et al., 2003a). The divergences in nucleotide sequence between individuals were calculated using the Kimura-two-parameter (K2P), which assumes distinct rates for both kinds of transition and transversions. This model is considered the best option for low distances ( Nei & Kumar, 2000; Hebert et al., 2003a). The distances were calculated using MEGA7 ( Kumar et al., 2016). Standard errors of the pairwise distances were calculated by 1000 bootstraps.