Eutyphoeus lokchaensis Tiwari & Yadav, 2025

Eutyphoeus lokchaensis Tiwari & Yadav sp. nov.

urn:lsid:zoobank.org:act:

Figs 7 View Fig , 14 View Fig ; Tables 1–2 View Table 1 View Table 2

Diagnosis

Length 125–185 mm, diameter 4.2–5 mm, segments 115–138. Prostomium tanylobic. First dorsal pore in 10/11. Male genital orifices bivestibulate, apenile, at ab left gonopore at a below the triangular, multi-fissured papillae. One pair of spermathecal pores in intersegment 7/8 at b or lateral to b. Genital markings post setal, median to aa, single in vii, viii, ix, x, xvi, presetal on xviii; postsetal, paired, at ab in vii, xvi; presetal, paired, at ab on viii, xviii; paired, postsetal, lateral to b. Septa all present. Intestinal caeca in xxvii, originated extremely lateral, ventrally directed. Holandric, seminal vesicle both present in ix and xii, posterior one extended up to xvi. Four-five pairs of supra-intestinal glands. Prostate extended up to xxii, duct very long, coiled, thick. Bidiverticulate spermathecae. Penial setae closely crowded circles of small fine triangular tooth-like ornamentation, slightly bent on anterior end, with blunt tip.

Etymology

The etymology of Eutyphoeus lokchaensis Tiwari & Yadav sp. nov. refers to its discovery location in the Yangoupokpi-Lokchao Wildlife Sanctuary, situated in Tengnoupal district, Manipur, India.

Material examined

Holotype

INDIA – Manipur • clitellate (few segments from caudal region taken for DNA extraction); Tengnoupal, Yangoupokpi-Lokchao Wildlife Sanctuary ; 24.3831° N, 94.1492° E; 10 Oct. 2017; Shweta Yadav leg.; registration number – ZSI CZRC T/9; MNP17-1075-44A1 . GoogleMaps

Paratypes

INDIA – Manipur • 3 clitellates; same data as for holotype; MNP17-1075-44A , GoogleMaps MNP17-1076-44A2 , GoogleMaps MNP17-1077-44A3 GoogleMaps .

Other material

INDIA – Manipur • 1 spec.; same data as for holotype; MNP17-1096-44A2 GoogleMaps .

Description

Holotype

Length 150 mm, diameter 4.2 mm. Total number of segments 136. Color cidar brown, uniformly pigmented. First dorsal pore starts from intersegmental furrow of 10/11. Prostomium tanylobic. Setae small, closely paired aa: ab: bc: cd: dd = 2.2: 1: 2.65: 1.42: 12.37 on xii, aa: ab: bc: cd: dd = 3.3: 1:2.29: 1.49: 12.89 on xxiv. Clitellum annular, covering ⅓ xiii to xvi segment. Spermathecal pore on intersegmental region of 7/8 at b or lateral to b, surrounded by fissured swollen lips with genital marking at lateral ends. Female pore on xiv, paired, presetal, slightly median to a, independent with or without common circle. Male genital region pentagonal in shape, male genital orifices bivestibulate, apenile, at ab left gonopore at a below triangular, multi-fissured papillae with narrow anterior end. Muscular composition of papilla compact. Genital markings post setal, median to aa, single in vii, viii, ix, x, xvi, presetal on xviii; postsetal, paired, at ab in vii, xvi; presetal, paired, at ab just below spermathecal pore on viii, xviii; paired, postsetal, lateral to b on surrounding surface of spermathecal pore. Median genital marking of xvi and xviii triangular in shape.

All septa present. Gizzard in viii segment. Intramural calciferous gland in xii. Intestine begins in xv. Lateral intestinal caeca in xxvii, originating extremely lateral, ventrally directed, conical with broad base and rounded tip. Last pair of hearts in xiii. Ventral intestinal caeca 7 in number, begins from xxxi–xxxviii. Typhlosole begins from xxvii. Supra-intestinal glands 4–5 pairs in segment lxxiv–lxxix. Holandric, testis sac annular in x and xi, seminal vesicles in ix and xii, posterior one extends up to xvi. Prostate in xvii, extended up to xxii, duct very long, coiled, thick. Spermathecae bidiverticulate, lateral, upwardly directed, multilobulated with 4–5 seminal chambers, duct very short or ductless, sac-like large ampulla. Penial setae two per battery, length 1.75–1.86 mm, width 0.039 mm, with closely crowded circles of small fine triangular tooth-like ornamentation, slightly bent on anterior end with blunt tip.

Variation

Overall, no substantial variations were detected.

Remarks

The species is classified within the hastatus group ( Gates 1958), and characterized by traits such as spermathecal pores positioned at b or lateral to b, bidiverticulate spermathecae, paired female pores, and holandric testes. It is distinguished by its vestibulated male genital orifices, a feature shared with E. tawi and E. marmoreus . However, the presence of lateral intestinal caeca sets it apart from E. tawi , while the presence of genital markings differentiates it from E. marmoreus ( Table 1 View Table 1 ).

DNA barcode

The holotype MNP17-1075-44A1 (process ID IEW1029-17) has been assigned the BIN number BOLD: ADL0070 based on COI barcode data. Eutyphoeus lokchaensis sp. nov. has a mean intraspecific divergence of 0.08% and a maximum intraspecific divergence of 0.15%. Its nearest neighbor species is Eutyphoeus lamdengensis sp. nov. with a nearest neighbor distance of 10.37 ( Table 2 View Table 2 ). All specimens of the novel species share the same BIN number.