Eutyphoeus shiroensis Tiwari & Yadav, 2025

Eutyphoeus shiroensis Tiwari & Yadav sp. nov.

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Figs 4 View Fig , 14 View Fig ; Tables 1–2 View Table 1 View Table 2

Diagnosis

Length 130 mm, diameter 4.7 mm, segments 125. First dorsal pore in 11/12. Male genital orifices in xvii avestibulate, apenile. One pair of spermathecal pores in intersegment 7/8 large at ab. Genital markings on xvi paired, postsetal at ab, also in xviii paired, postsetal at a. Septa 6/7/8/9 absent. Lateral intestinal caeca in xxviii. Holandric, seminal vesicles in xii segment extend up to xvii. 4 pairs of supra-intestinal glands. Prostate extends up to xxi, duct with one short coiled loop. Bidiverticulate spermathecae. Penial setae absent.

Etymology

The etymology of Eutyphoeus shiroensis Tiwari & Yadav sp. nov. is derived from its discovery in the Shiroi National Park, located in the northeastern state of Manipur, India. The specific epithet ‘ shiroensis ’ is derived from ‘Shiroi’, the name of the national park. This terminology intends to emphasize the significance of protecting the distinct ecosystems present in the park and to emphasize the necessity of preserving biodiversity in Manipur’s natural landscapes.

Material examined

Holotype

INDIA – Manipur • clitellate (2–3 segments from the tail region cut for DNA extraction); Shirui Kashong (Peak), Shiroi National Park ; 25.1302° N, 94.4202° E; elev. 2106.19 m; 11 Oct. 2017; Shweta Yadav leg.; registration number – ZSI CZRC T/15 ; MNP17-1104-45A1 . GoogleMaps

Description

Holotype

Length 130 mm, diameter 4.7 mm. The total number of segments 125 (excluding tail region aborted for molecular analysis). Prostomium (?). Color coffee brown, uniformly pigmented. First dorsal pore starts from intersegmental furrow of 11/12. aa: ab: bc: cd: dd = 2.04: 1: 2.04: 1.07: 10.24 on xii, aa: ab: bc: cd: dd = 3.51:1: 1.74: 1.83: 12.64 on xxiv. Clitellum pithos-shaped (terminals narrow and middle part enlarged in segment xv), covers four segments ½ xiii–⅓ xvii. Spermathecal pores in 7/8, at ab, large slit-like, body wall around pore swollen, fissured and swelling of pores merged mid ventrally without any demarcation, spermatophore present. Female pore paired, presetal on xiv slightly median to a. Male genital region beyond clitellum, male genital orifices avestibulate and apenile, on xvii under a hoof-like callus papillae at ab, each papilla extends laterally beyond ab. Paired and postsetal genital markings on xvi (at ab) and xviii (at a).

Septa 6/7/8/9 absent, 9/10 thickened. Gizzard in viii extends up to ix. Intramural calciferous gland in xii. Intestine begins in xv. Lateral intestinal caeca in xxviii. Typhlosole begins in xxviii. Ventral intestinal caeca 8 in number begins in xxxi. Four supra-intestinal glands pairs in 74–79. Last pair of hearts in xiv. Holandric, testis sacs in x and xi, annular; seminal vesicles in ix and xii with distinct hexagonal chambers, anterior one large petal-like in shape, posterior one extended up to xvii. Prostate in xvii extends up to xxi, duct short with one coiled loop. Spermathecae bidiverticulate, median and lateral overlapping each other, each diverticulum with two seminal chambers, ampulla large, oval-shaped with bulbous outgrowth on latero-anterior end, while ventrally no demarcation of bulging, ductless. Penial setae absent. Paired genital marking glands present in xvii, sessile.

Remarks

The novel species Eutyphoeus shiroensis is characterized by the paired spermathecal pores in segments vii/viii at ab, avestibulate male genital orifices, and holandric testes and thus classified within the hastatus group ( Gates 1958). It closely resembles E. phawngpuiensis , but is distinguished by the male genital orifices on xvii under hoof-like callus papillae at ab, each papilla extends laterally beyond ab, pithos-shaped clitellum, seminal vesicles with distinct hexagonal chambers in segments ix and xii, with the posterior vesicle extending to segment xvii, and the absence of penial setae ( Table 1 View Table 1 ).

DNA barcode

The COI barcode data for the holotype MNP17-1104-45A1, with process ID IEW1058-17, has been assigned the BIN number BOLD: ADL1719. For the newly described species, Eutyphoeus shiroensis sp. nov., the nearest neighbor species is Eutyphoeus tengnoupalensis Tiwari & Yadav sp. nov., with a nearest neighbor genetic distance of 10.94% ( Table 2 View Table 2 ).