Distoseptispora clematidis Phukhams., M. V. de Bult & K. D. Hyde

Distoseptispora clematidis Phukhams., M. V. de Bult & K. D. Hyde View in CoL , Fungal Diversity 102: 168 (2020)

Fig. 2 View Figure 2

Description.

Saprobic on dead branches in a terrestrial habitat. Sexual morph: Undetermined. Asexual morph: Hyphomycetous. Colonies on natural substrate effuse, scattered, dark brown to black, hairy. Mycelium partly superficial, partly immersed in the substratum, composed of branched, septate, smooth, pale brown to brown hyphae. Conidiophores macronematous, mononematous, unbranched, solitary or in groups, erect, straight or slightly flexuous, cylindrical, smooth, brown to dark brown, 2–5 - septate, robust at the base, 25–48.5 × 5–8 µm (x ̄ = 34.9 × 6.1 µm, n = 12). Conidiogenous cells monoblastic, integrated, terminal, cylindrical, pale brown to brown, smooth, flat at the conidiogenous loco. Conidia acrogenous, solitary, dry, obclavate, straight or curved, reddish-brown and slightly paler towards the apex, 26–40 - distoseptate, smooth, 150–270 × 11–15 µm (x ̄ = 195.1 × 12 µm, n = 30), tapering to 2.5–7.5 µm near the apex, 2.5–5.5 µm wide at the truncate base.

Culture characteristics.

Colonies on PDA reaching 76–80 mm diam. after 4 weeks in an incubator under dark conditions at 25 ° C, circular, surface velvety, with brown and denser mycelium at the center, becoming black at the margin with an obvious boundary, reverse black and sparser toward the boundary.

Material examined.

China • Jiangxi Province, Ganzhou City, Jiulianshan National Nature Reserve , 24°31'N, 114°27'E, on decaying wood of an unidentified broadleaf tree, 29 June 2022, Y. F. Hu ( HJAUP M 1319 , living culture HJAUP C 1319 ) GoogleMaps .

Notes.

Distoseptispora clematidis was introduced by Phukhamsakda et al. (2020), isolated from the dried stem of Clematis sikkimensis in Thailand. Phylogenetic analysis shows that our new isolate ( HJAUP C 1319 ) clusters with D. clematidis Phukhams., M. V. de Bult & K. D. Hyde ( MFLUCC 17–2145), with 100 % ML / 0.92 BI support. Morphologically, our isolate aligns well with the holotype description of D. clematidis . Comparisons of their nucleotide sequences showed 4 (0.7 %, including one gap), 3 (0.6 %, no gaps), and 2 (0.3 %, no gaps) nucleotide differences in the ITS, LSU, and RPB 2 regions, respectively, indicating that they are the same species.