Fusarium sulawesiense Sand.-Den., L. Lombard, Kema & Crous

Fusarium sulawesiense Sand.-Den., L. Lombard, Kema & Crous View in CoL [as ‘ sulawense’], Persoonia 43: 65 (2019)

Fig. 23 View Figure 23

Description.

Pathogenic to Mangosteen ( Garcinia mangostana ) and causes small, water-soaked lesions on the fruit surface; lesions may appear slightly sunken and surrounded by a yellow or brown halo. Sexual morph not observed. Conidiophores on aerial mycelium plentiful, septate, verticillately or irregularly branched. Conidiogenous cells mono- or polyphialidic, sub-cylindrical, smooth, thin-walled, 8–20 × 1.5–4 µm (mean = 14 × 3 μm, n = 20). Conidia on aerial mycelium falcate or fusiform, 1–5 septate: 1 - septate conidia 8.5–20 × 2.2–4.7 µm (mean = 13.5 × 3.5 μm, n = 30), 5 - septate conidia 32–42 × 2.5–4.5 µm (mean = 35 × 4.1 μm, n = 30). Sporodochia are formed on CLA after 7 days and have a pale orange color. Macroconidia in sporodochia falcate, with apical cells gently curved, papillate, and basal cells slightly curved, foot-shaped, 3–7 septate, 30–40.5 × 2.5–4 µm (mean = 33 × 3.6 μm, n = 30). Chlamydospores are absent.

Culture characteristics.

Colonies on PDA reach 65 mm in diameter after 7 days of growth at 25 ° C in the dark, cottony, white to pale pink, yellow, or pale brown in the center, and with a radial orange color on the reverse.

Material examined.

Thailand • Chiang Rai, Mueang Chiang Rai District, Ban Du , on fruit rot of Mangosteen ( Garcinia mangostana ), June 2023, Maryam Fallahi, dried culture L 5-6 ( MFLU 24-0253 ), living culture, MFLUCC 24-0250 .

Notes.

Based on phylogenetic analysis, MFLUCC 24-0250 clustered with Fusarium sulawesiense (InaCC F 940, holotype) in F. incarnatum species complex with 67 % ML, 97 % IQ bootstrap support, and 0.99 BYPP (Fig. 21 View Figure 21 ). The base pair differences between F. sulawesiense strains MFLUCC 24-0250 and the ex-type InaCC F 940 revealed a 0.36 % (2 / 554 bp) difference in tef 1 and no difference in rpb 2. The sequence of the rpb 1 gene is not available for the type strain. Fusarium sulawesiense ( MFLUCC 24-0250 ) is similar to the ex-type strain (InaCC F 964). However, the ex-type strain did not describe septate conidia ( Maryani et al. 2019 b). Fusarium sulawesiense was first reported from bananas in Indonesia ( Maryani et al. 2019 b). In Thailand, it was isolated from pineapple ( Abeywickrama et al. 2023), and this study provides the first host association of F. sulawesiense with Mangosteen ( Garcinia mangostana ).