Bartonella DNA

Detection of Bartonella DNA View in CoL in sand flies

All female sand flies were tested for Bartonella DNA by PCR on the basis of the gltA gene. Te results showed that 16 (2.87%) samples tested positive for Bartonella DNA ( Table 1). Of these, 13 samples were detected in Se. anodontis (12 samples) and Se. barraudi s.l. (1 sample) collected from Chiang Rai. Additionally, two samples of Se. anodontis (PT27-1 and PT250-17) infected with Bartonella sp. were trapped in Lampang while only one sample (SD39-8) was positive for Bartonella sp. in Se. khawi collected from Songkhla ( Table 1). On the basis of BLASTn analysis, the gltA sequences of all samples were close to Bartonella sp. (accession number KP100345) in the GenBank database with a range of 97.08–98.67% identities. ML tree analysis demonstrated the presence of a new Bartonella sp. that was closely related to Bartonella sp. isolated from bats as previously reported in Vietnam and Tailand (Supplementary 4: Fig. S3 View Fig ). Te gltA sequences were deposited in GenBank under the accession numbers OP903128-OP903143.