Nigrospora camelliae-sinensis Mei Wang & L. Cai

Nigrospora camelliae-sinensis Mei Wang & L. Cai View in CoL , in Wang, Liu, Crous & Cai, Persoonia 39: 127 (2017)

Fig. 2 View Figure 2

Description.

Endophytic from healthy leaves of Aquilaria sinensis . Sexual morph: Undetermined. Asexual morph: Hyphae branched, septate, hyaline, or light grey, 2–4 µm diam. Conidiophores 3–6 µm diam. micronematous, mononematous, solitary, smooth, branched or not, 0–1 septate, hyaline to subhyaline, consisting of 1–2 cells or usually reduced to conidiogenous cells. Conidiogenous cells 5.5–7.2 × 6.3–9.4 µm diam. x ̄ = 5.8 × 12.1 µm, n = 30), monoblastic, discrete, determinate, smooth, subglobose to ampulliform, subhyaline to light brown. Conidia 8–13 µm diam. x ̄ = 11 µm, n = 30) solitary, smooth-walled, aseptate, spherical, light grey to black, globose or subglobose.

Cultural characteristics.

Mycelium effusing on PDA within 12 hours from the edges of the surface sterilized leaf tissue piece. Colonies growing on PDA, hairy, black, reaching 9 cm in 7 days at 30 ° C; mycelium partly superficial, partly immersed, slightly effuse, radially striate, with an irregular edge, black. Asexual conidia spores were formed after 25 days on PDA. Sexual spores were not formed within 60 days.

Material examined.

China • Guangxi Zhuang Autonomous Region, Pubei City , in healthy living leaves of Aquilaria sinensis , September 25, 2020, Shiyu Zhang, GX 9-3 ( HKAS 134953 View Materials , new host record). Living culture KUNCC 23-16748 .

Known distributions

(based on molecular data): China ( Wang et al. 2017, this study).

Known hosts

(based on molecular data): Aquilaria sinensis (this study), Camellia sinensis ( Wang et al. 2017), Castanopsis sp. ( Wang et al. 2017), Musa paradisiaca ( Wang et al. 2017).

Notes.

Nigrospora camelliae-sinensis has been reported to have a cosmopolitan distribution and a broad host range ( Wang et al. 2017). In this study, our isolation ( KUNCC 23-16748 ), collected from Aquilaria sinensis in Guangxi Zhuang Autonomous Region, China, clustered in one single clade with N. camelliae-sinensis (94 % ML, 1 PP). Its morphological characteristics were in good agreement with those of N. camelliae-sinensis . Therefore, we regard this isolation ( KUNCC 23-16748 ) as N. camelliae-sinensis collected from Aquilaria sinensis for the first time. The study showed that N. camelliae-sinensis is also characterized by having determinate and micro- and mononematous, subcylindrical conidiophores.