Aedes aegypti

Aedes aegypti View in CoL adulticidal bioassay

The adulticidal bioassay was carried out according to the guidelines of the Centers for Disease Control and Prevention ( CDC, 2012). In the adulticidal bioassay, piperidyl dillapiole at concentrations of 1,000, 500, 250, 125 and 60 µg/mL and propyl ether dillapiole at concentrations of 600, 300, 150, 75 and 37 µg/mL, the dillapiole at 80 µg/mL used to compare with its derivatives, positive control with the insecticide Cielo ULV ® (Clarke Mosquito Control Products, Inc. Charles, IL 60174 USA) at 0.01 µg/mL (v/v), and the negative control solubilized in acetone 99.8% were impregnated on the walls in 250 mL bottles (Schott Duran®) the, in triplicate.After 12 hours, 10 fed females aged 3 to 5 days were transferred to each bottle, that allowed the entry of air, totaling 390 samples.Of the mosquitoes knocked out in each bottle, from time 0, every 15 min, up to 90 min, the percentage of mortality (immobility of movements) was calculated.The total number of dead individuals was divided by the total number at the beginning of the test and multiplied by 100.