Aedes aegypti

Aedes aegypti View in CoL larvicidal bioassays

The larvicidal bioassay with propyl ether dillapiole and piperidyl dillapiole was performed according to the recommendations of the World Health Organization ( WHO, 2005), with adaptations for the number of larvae and replications.A total of 600 larvae were used for both substances, 300 for the five concentrations of propyl ether dillapiole at 100, 50, 25, 12.5 and 6.25 µg/mL (v/v) and 300 for piperidyl dillapiole at 200, 100, 50, 25 and 12.5 µg/mL. The other larvae (n=180) were distributed dillapiole 80 µg/mL, used to compare with its derivatives, in the positive control (PC) temephos at 0.012 µg/mL, and in the NC, which was distilled water in DMSO 0.05%.The bioassay was performed in triplicate with 20 larvae per replica (n=60) in 20 mL of the solution of the substances .

After 24 hours of exposure, the number of dead larvae and those that did not respond to mechanical stimuli were counted and the mortality percentage was calculated to determine the larvicidal effect of dillapiole derivatives.