Aedes aegypti

Aedes aegypti View in CoL ovicidal bioassays

Ovicidal bioassays using propyl ether dillapiole and piperidyl dillapiole were performed according to Silva et al. (2019) and Oliveira et al. (2022), with adaptations. Of the total 780 eggs of Ae. aegypti , 600 were used for both substances; 300 for propyl ether dillapiole, at concentrations of 100, 50, 25, 12.5 and 6.25 µg/mL (v/v) and 300 eggs for piperidyl dillapiole at concentrations of 200, 100, 50, 25 and 12.5 µg/mL (v/v). The remaining eggs (n=180) were distributed in the dillapiole at 80 µg/ mL, used to compare with its derivatives, temephos – TM (Fersol 500 CE, Fersol Indústria e Comércio S/A, SP, Brazil) at 0.012 µg/mL (v/v) as positive control (PC), and the negative control (NC), which was distilled water in DMSO, Sigma Aldric, at 0.5% (v/v).

The bioassays were performed in triplicate with 20 eggs in each replica (n=60), and 10 mL of solution of each of the ten concentrations of both substances. After 4 h of exposure to the substances, the eggs were transferred to containers containing drinking water. Egg hatching was recorded 24 hours after the start of the bioassay.To determine the inhibition of viability of the eggs exposed to different concentrations of propyl ether dillapiole and piperidyl dillapiole, the percentage of unhatched eggs was calculated by dividing the number of hatched larvae by the total number of eggs multiplied by 10.