Torulaspora obscura, Silva & Paraíso & Al-Oboudi & Abegg & Aires & Barros & Brito & Jarzyna & Sylvester & Langdon & Opulente & Carriconde & Fell & Hofmann & Lachance & Legras & Libkind & Pontes & Gonçalves & Rosa & Groenewald & Hittinger & Sampaio, 2025

Torulaspora obscura f.a. M. Silva, F. Paraíso, C.A. Rosa & J.P. Sampaio, sp. nov. MB 853983 View Materials . Fig. 4X View Fig .

Etymology: Torulaspora obscura . ob.scu’ra, L. fem. adj. obscura, obscure, referring to the unknown natural niche of this species because all known strains have been isolated from anthropic environments.

Typus: Belgium, Oedelem, aquakefir, 2009, E. Vercammen (holotype PYCC 8933 View Materials H, ex-holotype cultures PYCC 8933 View Materials , CBS 18514 View Materials ). The holotype is permanently maintained in a metabolically inactive state in the Portuguese Yeast Culture Collection, Caparica, Portugal. The genome of this species was deposited at DDBJ /ENA/GenBank under the accession GCA_964263565. The version described in this paper is v. 1 .

Description: After 1 wk on YM agar at 25 °C, cultures are smooth, cream-coloured, and butyrous. After 3 d of growth on YM agar at 25 °C, cells are globose (2–4 µm) and occur singly or in pairs, and proliferation is by multilateral budding ( Fig. 4X View Fig ). On Dalmau plates after 2 wk at 25 °C, no pseudohyphae nor true hyphae are formed. Sexual reproduction was not observed on acetate agar or corn meal agar after prolonged incubation (3 mo) at 18 °C. The physiological and biochemical profile of the species in shown in Table 1 and Table S5.

Habitat and distribution: This species was found in anthropic environments, including aquakefir, orange soda and artificial orange juice. Currently known from collections in Belgium, UK and Brazil.

Additional cultures examined: NCYC 2473, PYCC 5189, UFMG-CM Y5068 (see Table S1 for details).