Torulaspora pahokee M. Silva, F. Paraíso, M. Groenewald, J.W. Fell & J.P. Sampaio, 2025

Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.

Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.