Torulaspora incommunis M. Silva, F. Paraíso, M. Groenewald & J.P. Sampaio, 2025

Silva, M. R., Paraíso, F., Al-Oboudi, J., Abegg, M., Aires, A., Barros, K. O., Brito, P. H., Jarzyna, M., Sylvester, K., Langdon, Q. K., Opulente, D. A., Carriconde, F., Fell, J. W., Hofmann, T. A., Lachance, M. - A., Legras, J. - L., Libkind, D., Pontes, A., Gonçalves, P., Rosa, C. A., Groenewald, M., Hittinger, C. T. & Sampaio, J. P., 2025, A taxogenomic view of the genus Torulaspora: an expansion from ten to twenty-two species, Persoonia 54 (1), pp. 265-283 : 276

publication ID	https://doi.org/10.3114/persoonia.2025.54.08
DOI	https://doi.org/10.5281/zenodo.16877636
persistent identifier	https://treatment.plazi.org/id/C549878B-7534-FFCB-FFD5-12F1A5408430
treatment provided by	Felipe
scientific name	Torulaspora incommunis M. Silva, F. Paraíso, M. Groenewald & J.P. Sampaio
status	sp. nov.

Treatment

Torulaspora incommunis M. Silva, F. Paraíso, M. Groenewald & J.P. Sampaio , sp. nov. MB 853859 View Materials . Fig. 4K, L View Fig .

Etymology: Torulaspora incommunis . in.com’mu.nis, L. fem. adj. incommunis, uncommon, referring to the apparent rarity of this species that, albeit first isolated more than 90 years ago, is known from a very low number of isolates.

Typus: Japan, Wakayama, sap of an orange tree ( Citrus sp. ), 1932, unknown collector (holotype PYCC 9318 View Materials H, ex-holotype cultures PYCC 9318 View Materials , CBS 5080 View Materials , IFO 0022 About IFO ). The holotype is permanently maintained in a metabolically inactive state in the Portuguese Yeast Culture Collection, Caparica, Portugal. The genome of this species was deposited at DDBJ /ENA/GenBank under the accession GCA_012851155. The version described in this paper is v. 1 .

Description: After 1 wk on YM agar at 25 °C, cultures are smooth, cream-coloured, and butyrous. After 3 d of growth on YM agar at 25 °C, cells are ellipsoidal (4–5 × 2.5–4 µm) and occur singly or in pairs, and proliferation is by multilateral budding ( Fig. 4K View Fig ). On Dalmau plates after 2 wk at 25 °C, no pseudohyphae nor true hyphae are formed. Sexual reproduction is observed on acetate agar after 20 d at 20 °C, and the cultures appear to be homothallic. Asci are persistent and are formed after cell-to-cell conjugation frequently involving a cell and its bud and occasionally two independent cells. Asci produce one to two smooth and spherical ascospores, measuring 2.5–3 µm diam. ( Fig. 4L View Fig ). The physiological and biochemical profile of the species in shown in Table 1 and Table S5.

Habitat and distribution: This species was found in the sap of an orange tree ( Citrus sp. ), Wakayama, Japan. Two additional cultures tentatively identified as this species were found in a rose flower in Fukuyama, Hiroshima, Japan and in soil in Taiwan.

Additional cultures examined: During the investigation of D1/ D2 and ITS sequences deposited at GenBank, two additional representatives of this species were presumptively detected (Table S2, Figs S2 View Fig , S 3 View Fig ): SG5S08, found in soil, Taiwan and FR 994 (NBRCN 114950), found in a rose flower, Fukuyama, Hiroshima, Japan ( Hisatomi & Toyomura 2021).