Torulaspora floroides M. Silva, F. Paraíso, K.O. Barros, C.A. Rosa & J.P. Sampaio, 2025

Torulaspora floroides M. Silva, F. Paraíso, K.O. Barros, C.A. Rosa & J.P. Sampaio , sp. nov. MB 853855 View Materials . Fig. 4E–G View Fig .

Etymology: Torulaspora floroides . flo.ro’i.des, N.L. fem. adj. floroides, flower-like, referring to the peculiar budding pattern seen in this species in which a central parent cell is surrounded by multiple smaller buds, thus resembling a flower.

Typus: Brazil, state of Amazonas, Itacoatiara, Amazonian rainforest, Campus of the Universidade Federal do Amazonas ( UFAM), rotting wood, 2019, K.O. Barros (holotype PYCC 9895 View Materials H, ex-holotype cultures PYCC 9895 View Materials , UFMG-CM-Y6992). The holotype is permanently maintained in a metabolically inactive state in the Portuguese Yeast Culture Collection, Caparica, Portugal. The genome of this species was deposited at DDBJ /ENA/GenBank under the accession GCA_964263535. The version described in this paper is v. 1 .

Description: After 1 wk on YM agar at 25 °C, cultures are smooth, cream-coloured, and butyrous. After 3 d of growth on YM agar at 25 °C, cells are globose (2.5–4 µm) to sub-globose (3–5 × 2.5–3 µm) and occur singly or in pairs, and proliferation is by conspicuous multilateral budding ( Fig. 4E View Fig ). On Dalmau plates after 2 wk at 25 °C, no pseudohyphae nor true hyphae are formed. Sexual reproduction is observed on acetate agar after 20 d at 20 °C, and the culture appears to be homothallic. Asci are persistent and normally are formed after conjugation involving a cell and its bud. Asci produce one to two smooth and spherical ascospores, measuring 2–3 µm diam. ( Fig. 4G View Fig ). Conspicuous multilateral budding resulting in a central parental cell surrounded by numerous buds with a flower-like appearance is observed in older cultures grown in sporulation medium ( Fig. 4F View Fig ). The physiological and biochemical profile of the species is shown in Table 1 and Table S5.

Habitat and distribution: This species was found only once, in rotting wood, Amazonas state, Brazil.