Torulaspora asahi M. Silva, F. Paraíso, M.-A. Lachance

Torulaspora asahi M. Silva, F. Paraíso, M.-A. Lachance &

J.P. Sampaio, sp. nov. MB 853717 View Materials . Fig. 4A–C View Fig .

Etymology: Torulaspora asahi . a.sa.hi’, N.L. app. n. asahi, the Japanese word “asahi ” meaning rising sun Japan’s sobriquet (“the Land of the Rising Sun”) as several strains of this species, including the ex-type strain, were isolated in that country.

(A) (B) (C)

Zygotorulaspora mrakii 44 46 214 284 285 594

T. delbrueckii PYCC 2477T DEL C A T G G T

T. mapucheana PYCC 9893

MAP T T C A C -

T. pahokee CBS 11100T PAH CUK PRE FRA 33 62 67 113 184 189 225 258 631 696 698

T. cukson UWOPS 85-398.1-1T 6 PAH C G C - A T T C A A C CUK C A T - A T C C G G T

T. pretoriensis NRRL Y-17251T 10 4 PRE T A T C C A C T G G T

T. franciscae CBS 2926 T 14 12 16

T. incommunis CBS 5080T 20 18 16 23

64 65 120 278 289 684

T. markjohnstonii MAR G A C A G -

T. asahi PYCC 8100 ASA A G G G A A

T. indica CBS 12408

T. ventriculi PYCC

T. globosa CBS 764

T. kanakia PYCC 8417 T

T. jiuxiensis CBS 16004 T

T. nypae TBRC 10639 T

T. gathmanii PYCC 9889

T. maleeae CBS 10694 T

T. floroides UFMG-CM-Y

T. quercuum CBS 11403 T

T. nezacadeziaeae PYCC

T. obscura PYCC 8933T

20 T. microellipsoides NRRL Y-1549

Tree scale: 0.1

Typus: Japan, Shiba Prefecture , soil underneath Quercus acuta , 2008, Y. Imanishi (holotype PYCC 8100 View Materials H, ex-holotype cultures PYCC 8100 View Materials , CBS 18509 View Materials ). The holotype is permanently maintained in a metabolically inactive state in the Portuguese Yeast Culture Collection, Caparica, Portugal. The genome of this species was deposited at DDBJ /ENA/ GenBank under the accession GCA_931301635. The version described in this paper is v. 1 .

Description: After 1 wk on YM agar at 25 °C, cultures are smooth, cream-coloured, and butyrous. After 3 d of growth on YM agar at 25 °C, cells are globose (4–5.5 µm) to sub-globose (4–6 × 3–5 µm) and occur singly or in pairs, and proliferation is by multilateral budding ( Fig. 4A View Fig ). On Dalmau plates after 2 wk at 25 °C, no pseudohyphae nor true hyphae are formed. Sexual reproduction is observed on acetate agar after 15 d at 20 °C, and the cultures appear to be homothallic. Cellular extensions that resemble conjugation tubes are frequent and usually are not involved in conjugation ( Fig. 4B View Fig ). Asci are persistent and normally are formed after conjugation involving a cell and its bud. Asci produce one to two smooth and spherical ascospores, measuring 2.5–3.5 µm diam. ( Fig. 4C View Fig ). The physiological and biochemical profile of the species is shown in Table 1 and Table S5.

Habitat and distribution: This species was found in the arboreal niche, including soil underneath Quercus acuta and Q. salicina , bark of Lithocarpus edulis , root of Clermontia sp. , exudate of Myoporum , and fruit of Ficus virgata . Currently known from collections in Japan (Chiba Prefecture and Iriomote island) and Hawaii.

Additional cultures examined: PYCC 8099, PYCC 8101, PYCC 9382, UWOPS 91-901.1, UWOPS 91-920.1 (see Table S1 for details).