Nigrospora oryzae (Berk. & Broome) Petch

Nigrospora oryzae (Berk. & Broome) Petch View in CoL , J. Indian bot. Soc. 4: 24 (1924)

Fig. 4 View Figure 4

Monotospora oryzae Berk. & Broome View in CoL , J. Linn. Soc., Bot. 14: 99. 1873. Basionym.

Khuskia oryzae H. J. Huds. View in CoL , Trans. Brit. Mycol. Soc. 46: 358. 1963. Synonym.

Description.

Endophytic from healthy leaves of Aquilaria sinensis . Sexual state: Undetermined. Asexual state: Hyphomycetes. Hyphae 1–3.5 µm diam. branched, smooth, septate, hyaline, or subhyaline. Conidiophores 2–5 µm diam. micronematous, solitary in sporodochia, subcylindrical, pale grey, smooth, 0–2 - septate, branched or not, usually reduced to conidiogenous cells. Conidiogenous cells 4.7–5.2 × 6.3–7.4 µm diam. x ̄ = 5.0 × 6.9 µm, n = 50), monoblastic, solitary, determined, hyaline to subhyaline, ampliform to subspherical, discrete, smooth-walled. Conidia 8–13.5 µm diam x ̄ = 11.3 µm, n = 50) solitary, globose or subglobose, black, shiny, smooth, aseptate.

Cultural characteristics.

Mycelium effusing on PDA within 12 hours from the edges of the surface-sterilized leaf tissue piece. Colonies growing on PDA, hairy, black, reaching 5 cm in 7 days at 30 ° C; mycelium partly superficial, partly immersed, slightly effuse, radially striate, with an irregular edge, dark brown to black colored; asexual spores were formed after 25 days on PDA, and sexual spores were not formed within 60 days on PDA.

Material examined.

China • Guangxi Zhuang Autonomous Region, Pubei city , on healthy living leaves of Aquilaria sinensis , September 25, 2020, Shiyu Zhang, GX 2-3 ( HKAS 134951 View Materials , new host record). Living culture KUNCC 23-16746 .

Known distribution

(based on molecular data): Australia ( Barkat et al. 2016), Bangladesh ( Begum et al. 2018), China ( Sun et al. 2011; Chen and Kirschner 2018; He et al. 2019; Zhang et al. 2019, this study), Iran ( Hashemian et al. 2014), Italy ( Lorenzini et al. 2016), Kazakhstan ( Eken et al. 2016), Pakistan ( Alam et al. 2017), Sri Lanka ( Wang et al. 2017), and Thailand ( Hyde et al. 2020).

Known hosts

(based on molecular data): Acer truncatum ( Sun et al. 2011), Actinidia sp. ( Li et al. 2018), Aloe vera ( Alam et al. 2017), Aquilaria sinensis (This study), Castanopsis sp. ( Wang et al. 2017), Cephalotaxus sinensis ( Wang et al. 2017), Citrullus lanatus ( Chen and Kirschner 2018), Citrus reticulata ( Wang et al. 2017), Cleyera japonica ( Wang et al. 2017), Daphniphyllum macropodum ( Wang et al. 2017), Daphniphyllum oldhamii ( Wang et al. 2017), Gossypium hirsutum ( Zhang et al. 2019), Hamamelis mollis ( Wang et al. 2017), Nelumbo nucifera ( Chen and Kirschner 2018), Nelumbo sp. ( Wang et al. 2017), Neolitsea sp. ( Wang et al. 2017), Oryza sativa ( Wang et al. 2017), Osmanthus fragrans ( Wang et al. 2017), Osmanthus sp. ( Wang et al. 2017), Pennisetum americanum ( Hashemian et al. 2014), Pentactina rupicola ( Wang et al. 2017), Photinia serrulata ( He et al. 2019), Phyllostachys heterocycla ( Hyde et al. 2020), Rhododendron simiarum ( Wang et al. 2017), Rhododendron sp. ( Wang et al. 2017), Rubus reflexus ( Wang et al. 2017), Rubus sp. ( Wang et al. 2017), Symplocos zizyphoides ( Wang et al. 2017), Ternstroemia sp. ( Wang et al. 2017), Triticum aestivum ( Eken et al. 2016; Barkat et al. 2016), Tutcheria microcarpa ( Wang et al. 2017), Vaccinium corymbosum ( Zhang et al. 2019), Vitis vinifera ( Lorenzini et al. 2016).

Notes.

Nigrospora oryzae has been reported to have a cosmopolitan distribution and a broad host range ( Wang et al. 2017). In this study, our isolate ( KUNCC 23-16746 ) clustered in the same clade (100 % ML, 1 PP) as N. oryzae , and its morphological characteristics were consistent with those of N. oryzae ( Wang et al. 2017) . Therefore, we regard this isolate ( KUNCC 23-16746 ) as N. oryzae , which was collected from Aquilaria sinensis in the Guangxi Zhuang Autonomous Region, China, for the first time.