Chlorocillium guizhouense W. H. Chen, Y. F. Han & J. D. Liang, 2025

Chlorocillium guizhouense W. H. Chen, Y. F. Han & J. D. Liang sp. nov.

Fig. 5 View Figure 5

Etymology.

Referring to the location, Guizhou Province, where the type specimen was collected.

Type.

China • Guizhou Province, Qiandongnan Miao and Dong Autonomous Prefecture, Rongjiang County, Dali Dong Village (26°01'58.70"N, 108°24'48.06"E). On a dead spider ( Araneae ), 1 October 2018, Wanhao Chen, GZAC DL 1017 (holotype), ex-type, DL 10171 GoogleMaps .

Description.

Colonies on PDA reaching 21–24 mm diam. in 14 d at 25 ° C, white, consisting of a basal felt and cottony, floccose overgrowth, reverse yellowish. Prostrate hyphae smooth, septate, hyaline, 1.1–1.6 μm diam. Conidial structures consisting of erect conidiophores usually arising from the aerial hyphae, solitary or lecanicillium-like with phialides in whorls of two to three. Phialides 11.6–25.3 × 1.0–1.2 μm, with a cylindrical basal portion, tapering into a distinct neck. Conidia in chains, hyaline, fusiform, 1 - celled, 2.6–3.8 × 1.1–1.6 μm (average values 3.2 × 1.3 μm). Octahedral crystals not observed.

Host.

Spider ( Araneae ).

Additional material examined.

China • Guizhou, Qiandongnan Miao and Dong Autonomous Prefecture, Rongjiang County, Dali Dong Village (26°01'58.70"N, 108°24'48.06"E). On a dead spider ( Araneae ), 1 October 2018, Wanhao Chen, DL 10172 (living culture) GoogleMaps .

Remarks.

Chlorocillium guizhouense was identified as Chlorocillium , based on the BLASTn result in NCBI and the phylogenetic analysis of the combined dataset 1 (Fig. 1 View Figure 1 ). It clustered into an independent clade with a close relationship with C. araneogenum , C. sinense and C. vallense with high bootstrap value (93 % ML). Compared with the typical characteristics of the known species (Table 2 View Table 2 ), C. guizhouense was distinguished from C. araneogenum by its smaller conidia (2.6–3.8 × 1.1–1.6 μm vs. 3.4–5.8 × 1.4–1.8 μm). Chlorocillium guizhouense can be distinguished from C. sinense by its larger conidia (fusiform, 2.6–3.8 × 1.1–1.6 μm vs. fusiform to ellipsoidal, 1.9–2.9 × 0.8–1.2 μm). Chlorocillium guizhouense was distinguished from C. vallense by its larger phialides (11.6–25.3 × 1.0–1.2 μm vs. 9.2–17.1 × 0.8–1.2 μm). Thus, the morphological characteristics and molecular phylogenetic results support C. guizhouense as a new species.