Carlosrosaea wuzhiensis W. L. Gao & F. L. Hui, 2025

Carlosrosaea wuzhiensis W. L. Gao & F. L. Hui sp. nov.

Fig. 2 E, F View Figure 2

Etymology.

The specific epithet “ wuzhiensis ” refers to the geographic origin of the type strain of the species, Wuzhi Mountain.

Type.

China • Hainan Prov.: Sanya City, Wuzhi Mountain , 32°45'N, 113°30'E, in the phylloplane of Glochidion zeylanicum (Gaertn.) A. Juss , 15 Aug 2024, S. L. Lv, NYNU 24841 (holotype GDMCC 2.526 T, preserved in a metabolically inactive state; culture ex-type PYCC 10136 View Materials , preserved in a viable metabolically inactive state; GenBank: PQ 568987 , PQ 568982 ) GoogleMaps .

Description.

On YM agar after 7 days at 20 ° C, the streak culture is pale-yellow, butyrous, smooth, and glossy, with an entire margin (Fig. 2 E View Figure 2 ). After 3 days in YM broth at 20 ° C, cells are ovoid and ellipsoidal, 2.4–3.8 × 3.8–6.9 μm, and single; budding is polar (Fig. 2 F View Figure 2 ). After 1 month at 20 ° C, a ring and sediment are present. In Dalmau plate culture on CMA, pseudohyphae and hyphae are not formed. Sexual structures are not observed on PDA, CMA, or V 8 agar. Ballistoconidia are not produced. Glucose fermentation is absent. The following compounds are assimilated as sole carbon sources: glucose, sucrose (weak), raffinose, melibiose (weak), galactose (weak), lactose (delayed and weak), trehalose (weak), maltose (weak), melezitose (weak), methyl-α-D-glucoside (delayed and weak), cellobiose, salicin (weak), L-sorbose (delayed and weak), L-rhamnose (delayed and weak), D-xylose (weak), L-arabinose, D-arabinose, 5 - keto-D-gluconate, D-ribose (weak), glycerol (delayed and weak), ribitol, galactitol (delayed and weak), D-mannitol (delayed and weak), D-glucitol (delayed), myo-inositol (delayed and weak), DL-lactate (delayed and weak), succinate (weak), citrate, D-gluconate (weak), D-glucosamine (delayed and weak), N-acetyl-D-glucosamine (weak), 2 - keto-D-gluconate (delayed and weak), D-glucuronate (weak), and glucono- 1, 5 - lactone. Inulin, methanol, ethanol, and erythritol are not assimilated. Nitrate (delayed and weak), nitrite (delayed and weak), and L-lysine are assimilated as sole nitrogen sources. Ethylamine and cadaverine are not assimilated. Maximum growth temperature is 30 ° C. Growth on 50 % (w / w) glucose-yeast extract agar is negative. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive.

Additional strain examined.

China • Hainan Prov.: Sanya City, Wuzhi Mountain , 32°45'N, 113°30'E, in the phylloplane of Glochidion zeylanicum , 15 Aug 2024, S. L. Lv, NYNU 248116 GoogleMaps .

Note.

Phylogenetic analyses indicate that the closest known species to C. wuzhiensis sp. nov. is C. yunnanensis . Both form pale-yellow colonies and produce ovoid to ellipsoidal cells. However, C. wuzhiensis can be distinguished from C. yunnanensis by its ability to assimilate cellobiose, L-sorbose, D-arabinose, glycerol, galactitol, and citrate, and its inability to assimilate inulin and erythritol. Additionally, C. wuzhiensis can grow in a vitamin-free medium and at 25 ° C, whereas C. yunnanensis cannot (Table 2 View Table 2 ).