Daldinia eschscholtzii (Ehrenb.) Rehm

Daldinia eschscholtzii (Ehrenb.) Rehm View in CoL , Annls. Mycol. 2 (2): 175 (1904)

Fig. 4 View Figure 4

Description.

Endophytic from healthy panicle of Oryza sativa . Sexual morph: not observed. Asexual morph: hyphomycetous. Conidiophores 1–2.3 × 0.9–1.7 µm (x ̄ = 2 × 1.4 µm, n = 10), mononematous, dichotomously or trichotomously branched, with Nodulisporium - like branching pattern, bearing 1–3 conidiogenous cells from each whorl, hyaline. Conidiogenous cells 2.6–4 × 1.5–4 µm (x ̄ = 3.5 × 2 µm, n = 10), holoblastic, terminal or intercalary, cylindrical, with rounded apices, hyaline. Conidia 3–5.8 × 2.6–4.2 µm (x ̄ = 4 × 3.3 µm, n = 30), smooth, obovoid to ellipsoid, aseptate, mostly with flat base, hyaline.

Culture characteristics.

Colonies on PDA reaching 63–65 mm in diameter after 7 days at 27 ° C, initially white with a diffuse margin. Becoming grayish olive green with dull green patches; reverse black at the center and whitish gray at the periphery.

Material examined.

Thailand • Chiang Rai Province, Phan District , from healthy tissue of rice panicle, 25 October 2021, Nootjarin Jungkhun (NS 11-1 a = MFLU 25-0025 View Materials ); (living culture MFLUCC 24-24-0504 ) .

GenBank numbers.

MFLUCC 24-0504 : ITS = PV 235051, LSU = PV 235061, rpb 2 = PV 275678, tub 2 = PV 275684; MFLUCC 24-0505 : ITS = PV 235052, LSU = PV 235062, rpb 2 = PV 275679, tub 2 = N / A; MFLUCC 24-0506 : ITS = PV 235053, LSU = N / A, rpb 2 = PV 275680, tub 2 = N / A.

Notes.

Based on the morphological and molecular data, all three strains ( MFLUCC 24-0504 , MFLUCC 24-0505 , and MFLUCC 24-0506 ) were identified as Daldinia eschscholtzii with 99 % ML and 0.99 PP bootstrap support (Fig. 5 View Figure 5 ). Isolates MFLUCC 24-0504 , MFLUCC 24-0505 , and MFLUCC 24-0506 were obtained from panicles of both glutinous and jasmine rice collected from various locations in Chiang Rai Province of Thailand (Table 2 View Table 2 ).