Bonnetina alagoni, LOCHT & MEDINA, 2008

BONNETINA ALAGONI LOCHT & MEDINA, 2008 View in CoL

( FIGS 1, 2, 5A, 10B; TABLE 1)

urn:lsid:zoobank.org:act:C34CD7E3-D7D8-435B-AFD9-39E9826AA99D

Bonnetina alagoni Locht & Medina, 2008: 45 View in CoL , figs. 1–8. Estrada-Alvarez & Locht, 2011: 154, figs 14, 17.

Type locality: MEXICO: Morelos State: Tepoztlán municipality: Tepoztlán (19.01°, –99.06°) .

Material examined (n = 10): MEXICO: Morelos State: Tepoztlán municipality. ♂ a holotype ( CNAN-T0736 ) and ♀ paratype ( CNAN-T0737 ). Tepoztlán: 19.01°, –99.06°. 5/ II /2007. Rafael Aguilar Cortés, col. ♂ a (CNAN-Ar3650A) and 2 ♀♀ a (CNAN-Ar3650B, CNANAr3650C): 1 km east of San Juan Tlacotenco town : 19.0178°, –99.0811 °; 2420 masl. 13/IV/2012. David Ortiz, Diego Barrales, Jorge Mendoza, Gerardo Contreras, Carlos Santibáñez, Laura Olguín and Natalia Zepeda, cols. Under stones. 3 ♀♀ a (CNAN-Ar10094A-C): 1 km east of San Juan Tlacotenco town : 19.0199°, –99.0798 °; 2400 masl. 20/ II /2015. D. Ortiz and Ricardo Paredes, cols. In shallow burrows under stones. Two specimens (A and B) with egg sacs. Secondary vegetation. ♂ a (CNAN-Ar4099A) and ♀ a (CNAN-Ar4099B). Tepoztlán : 19.01°, –99.06 °; 2200 masl. 11/ III /1993. George Odell and Rick C. West, cols. Under stones .

Diagnosis (emended): Morphology and natural history. Males differ from those of most Bonnetina species by having poorly developed tibiae I accessory apophysis, not geniculate pedipalpal bulbs, and moderately long and sub-conical bulbal embolus, with PSA keel. It differs from B. juxtantricola by having the tibia I retrolateral apophysis tip rounded (instead of conical), by having a poorly developed but distinct tibia I accessory apophysis and by the pedipalpal bulb shape. Females ( Fig. 10B) differ from those of most species by having domiform-low spermatheca, sternum sub-circular (instead of sub-oval) and urticating hair patch normal (instead of reduced). They differ from females of B. aviae by reaching larger sizes, and by being known only from one locality in northern Morelos, whereas B. aviae is known from the surroundings of the Valley of Mexico and one locality in Veracruz. DNA. Diagnostic COI nucleotides (5): 84 (G), 306 (G), 519 (G), 822 (C), 885 (C). COI p-distances to other species above 6.5%; intra-specific distances less than 2.5% (Appendices S1, S5).

Species delimitation methods: Morphology, HG barcoding and PTP.

Genetic diversity. COI: KU664207 View Materials - KU664211 View Materials ( Fig. 2; Appendix S 1) . Intra-specific variation <2.2%.

Distribution and natural history: Bonnetina alagoni is known only from north of Tepoztlán, in Morelos, between 2200 and 2500 masl (Trans-Mexican Volcanic Belt) ( Fig. 1; Table 1). The distribution area is surrounded by oak forest, but we have only collected the species in exposed areas with disturbed vegetation ( Fig. 5A), in shallow burrows under stones. Adult males have been collected between February and April and females also seem to be highly seasonal: whereas a few trips out of season failed to find any specimens, on 20 February 2015, we located about 15 adult females (most of them with cocoons) in a previously explored spot of about 100 m 2. Such contrast in the abundance of specimens makes us to suspect that the specimens plug their burrows during unfavourable conditions.