Chlamydosporoides sinensis H. Pan & Zhi. Y. Zhang, 2025

Chlamydosporoides sinensis H. Pan & Zhi. Y. Zhang sp. nov.

Fig. 2 View Figure 2

Etymology.

The epithet “ sinensis ” (Lat.) refers to China, where the species was collected.

Type.

China: • Guizhou Province, Guiyang City, Huaxi District, Guizhou Minzu University , 26°46'25"N, 106°66'95"E, from epiphytic soil of Camphora officinarum Boerh. ex Fabr. , 25 July 2024, Heng Pan and Zhi-Yuan Zhang ( holotype HMAS 354106 , dried culture; ex-type CGMCC 3.29099 = ZY 24.003 ; ibid. ZY 24.004 ) .

Description.

Culture characteristics (14 days at 25 ° C): Colony on PDA 39–43 mm diam. flat, agate gray to platinum gray from center to margin, nearly circular, margin regular; reverse: light ivory to oyster white from center to margin. Colony on OA 30–34 mm diam. flat, graphite gray to green beige from center to margin, nearly circular, margin regular; reverse: dark ivory. Colony on SNA 53–58 mm diam. fluffy, hyphae sparse, honey yellow; reverse: honey yellow.

Hyphae branched, septate, hyaline to brown, smooth, 1–4 μm wide. sometimes hyphopodia-like structures creating a cauliflower-like appearance. Conidiogenous cells smooth, solitary, cylindrical to clavate, straight or curved, aseptate, 13–15.5 × 2.5–3.5 μm. Conidia chlamydospores-like, solitary, hyaline to brown, smooth, non-septate, pyriform, globose to ellipsoid, or irregular shapes, sessile or borne on conidiogenous cells, 6–12 × 5–7 μm (av. 8.2 × 6.1, n = 50). Chlamydospores borne on hyphae, solitary, catenate, or grape-like, brown to dark brown, 11–17.5 × 7.5–13 μm (av. 12.8 × 10, n = 30). Setae, Conidiomata, and Sexual morph unknown.

Geographical distribution.

Guizhou Province, China.

Additional material examined.

China: • Guizhou Province, Guiyang City, Huaxi District, Guizhou Minzu University , 26°46'15"N, 106°66'81"E, from epiphytic soil of Camphora officinarum , 25 July 2024, Heng Pan and Zhi-Yuan Zhang ZY 24.005 , ibid. ZY 24.006 ) .

Notes.

Phylogenetic analysis revealed that four new isolates ( ZY 24.003 –24.006) formed a distinct subclade within Chlamydosporoides with strong statistical support (100 / 0.97, Fig. 1 View Figure 1 ). Morphologically, Chlamydosporoides sinensis differs from C. guizhouensis by its smooth-walled conidia and the production of abundant chlamydospores. Furthermore, these species exhibit significant molecular differentiation, as evidenced by sequence comparisons of three loci: C. sinensis (ex-type CGMCC 3.29099 ) shows 91.6 % ITS (550 / 600 bp, 13 gaps), 98.3 % LSU (839 / 853 bp, one gap), and 95.9 % tef 1 (892 / 930 bp, one gap) similarity when compared with C. guizhouensis (ex-type CGMCC 3.29100 ). Due to the lack of original descriptions for strains CBS 101058 and CBS 101143 (designated as ‘ W. laurinus ’), we restricted our comparison to sequence analyses. CBS 101058 demonstrated 97.6 % ITS (527 / 540 bp, 2 gaps) and 100 % LSU (834 / 834 bp, no gaps) similarity with C. sinensis ( CGMCC 3.29099 ), suggesting potential conspecificity. In contrast, CBS 101143 showed markedly lower similarity (76.3 % ITS [449 / 588 bp, 51 gaps] and 97.8 % LSU [816 / 834 bp, one gap]) relative to the C. sinensis ex-type strain.