Hesperia pahaska hidalgo Grishin

Hesperia pahaska hidalgo Grishin , new subspecies

http://zoobank.org/ 1ADE0404-E9CA-4E0B-9003-A0A325B6CDBD

( Figs. 126 View Fig part, 128 part, 129)

Definition and diagnosis. Genomic analysis of two specimens of Hesperia pahaska Leussler, 1938 (type locality in USA: Nebraska, Sioux Co.) from Hidalgo, Mexico, places them separately from other populations in a clade genetically differentiated at least at the subspecies level ( Fig. 126 View Fig ); e.g., their COI barcodes differ from geographically closest Hesperia pahaska tehaska ssp. n. by 1.7% (11 bp), and, therefore, represent a new subspecies. This new subspecies keys to “ Hesperia columbia pahaska ” (M.10.5.(b)) in Evans (1955) and differs from other subspecies of H. pahaska by being smaller, darker, especially on the ventral hindwing, with larger submarginal pale spots near the forewing apex and redder, not greenish or yellowish, tones of the ventral side of wings, and by submarginal spots in forewing cells M 1 -M 2 and M 2 -M 3 being longer and reaching closer to the wing outer margin. Due to the cryptic nature of this subspecies and unexplored individual variation, most reliable identification is achieved by DNA, and a combination of the following base pairs is diagnostic in the nuclear genome: aly838.4.2:G156T, aly838.4.2:G159A, aly839.15.3:G75A, aly839.15.3:A76C, aly613.3.6: A141G; and COI barcode: T250C, C282T, G389A, T485C, A625G.

Barcode sequence of the holotype. Sample NVG- 23049G08, GenBank PV550056, 658 base pairs:

Type material. Holotype: ♂ deposited in the McGuire Center for Lepidoptera and Biodiversity Collection, Gainesville, FL, USA ( MGCL), illustrated in Fig. 129 View Fig , bears the following six rectangular labels (1 st handwritten, others printed), five white: [ MEXICO. Hidalgo: | Rt.85, 90.4 mi | N. Pachuca , 4- Aug-1981 | leg. Douglas Mullins], [ Hesperia | pahaska williamsi | Lindsey | Det. W.W. McGuire], [Collection of | William W. McGuire], [FSCA | Florida State Collection | of Arthropods], [DNA sample ID: | NVG-23049G08 | c/o Nick V. Grishin ], and one red [HOLOTYPE ♂ | Hesperia pahaska | hidalgo Grishin ]. Paratype: 1♂ NVG-24097G03 with the same data as the holotype.

Type locality. Mexico: Hidalgo, Rt. 85, 90.4 mi north of Pachuca .

Etymology. The name of the state with the type locality is used as the name of the new subspecies and is treated as a noun in apposition.

Distribution. Currently known only from the state of Hidalgo in Mexico.